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Conversion of 7-Carboxy-Cannabidiol (7-COOH-CBD) to 11-Nor-9-Carboxy-Tetrahydrocannabinol (THC-COOH) during Sample Preparation for GC–MS Analysis
Journal of Analytical Toxicology ( IF 2.5 ) Pub Date : 2021-05-13 , DOI: 10.1093/jat/bkab046
E Dale Hart 1 , Svante Vikingsson 1 , John M Mitchell 1 , Ruth E Winecker 1 , Ronald Flegel 2 , Eugene D Hayes 2
Affiliation  

The growing use of cannabidiol (CBD) products by the general public is expected to result in an increase in the prevalence of CBD and the CBD metabolites in drug testing laboratories. CBD converts into tetrahydrocannabinol (THC) under acid conditions which could produce false-positive results, but little is known about how the presence of the urinary metabolite of CBD, 7-carboxy-cannabidiol (7-COOH-CBD), would affect urine drug testing for 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH). As the operators of the National Laboratory Certification Program (NLCP), we prepared a set of performance testing samples containing 7-COOH-CBD for cannabinoid testing at the laboratories accredited by the NLCP to investigate if 7-COOH-CBD can produce false-positive results for THC-COOH during immunological screening analysis and if 7-COOH-CBD can be converted to THC-COOH. At concentrations up to 2,500 ng/mL, 7-COOH-CBD was not reactive by immunoassay in any of the four different immunoassay kits used. Additionally, we did not observe any significant conversion of 7-COOH-CBD to THC-COOH in assays used by NLCP-certified laboratories. However, we did see conversion when we requested that selected laboratories retest their samples using derivatization with perfluorinated anhydrides in combination with perfluorinated alcohols or when samples containing 7-COOH-CBD were exposed to acid for an extended time.

中文翻译:

在 GC-MS 分析样品制备过程中将 7-羧基-大麻二酚 (7-COOH-CBD) 转化为 11-Nor-9-羧基-四氢大麻酚 (THC-COOH)

公众对大麻二酚 (CBD) 产品的使用日益增加,预计将导致药物检测实验室中 CBD 和 CBD 代谢物的流行率增加。CBD 在酸性条件下会转化为四氢大麻酚 (THC),这可能会产生假阳性结果,但人们对 CBD 的尿液代谢物 7-羧基大麻二酚 (7-COOH-CBD) 的存在如何影响尿液药物知之甚少测试 11-nor-9-羧基-四氢大麻酚 (THC-COOH)。作为国家实验室认证计划(NLCP)的运营商,我们准备了一组包含 7-COOH-CBD 的性能测试样品,用于在 NLCP 认可的实验室进行大麻素测试,以调查 7-COOH-CBD 在免疫筛查分析期间是否会产生 THC-COOH 的假阳性结果,以及是否 7- COOH-CBD 可以转化为 THC-COOH。在高达 2,500 ng/mL 的浓度下,7-COOH-CBD 在所使用的四种不同免疫测定试剂盒中的任何一种中都没有通过免疫测定反应。此外,在 NLCP 认证实验室使用的分析中,我们没有观察到 7-COOH-CBD 到 THC-COOH 的任何显着转化。但是,当我们要求选定的实验室使用全氟酸酐和全氟醇的衍生化方法重新测试他们的样品时,或者当含有 7-COOH-CBD 的样品长时间暴露于酸时,我们确实看到了转化。在高达 2,500 ng/mL 的浓度下,7-COOH-CBD 在所使用的四种不同免疫测定试剂盒中的任何一种中都没有通过免疫测定反应。此外,在 NLCP 认证实验室使用的分析中,我们没有观察到 7-COOH-CBD 到 THC-COOH 的任何显着转化。但是,当我们要求选定的实验室使用全氟酸酐和全氟醇的衍生化方法重新测试他们的样品时,或者当含有 7-COOH-CBD 的样品长时间暴露于酸时,我们确实看到了转化。在高达 2,500 ng/mL 的浓度下,7-COOH-CBD 在所使用的四种不同免疫测定试剂盒中的任何一种中都没有通过免疫测定反应。此外,在 NLCP 认证实验室使用的分析中,我们没有观察到 7-COOH-CBD 到 THC-COOH 的任何显着转化。但是,当我们要求选定的实验室使用全氟酸酐和全氟醇的衍生化方法重新测试他们的样品时,或者当含有 7-COOH-CBD 的样品长时间暴露于酸时,我们确实看到了转化。
更新日期:2021-05-13
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