当前位置: X-MOL 学术Sci. Total Environ. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Maternal Zearalenone Exposure Impacted Ovarian Follicle Formation and Development of Suckled Offspring
Science of the Total Environment ( IF 9.8 ) Pub Date : 2021-05-16 , DOI: 10.1016/j.scitotenv.2021.147792
Li Kong 1 , Ai-Hong Zhao 2 , Qian-Wen Wang 3 , Yan-Qin Feng 1 , Zi-Hui Yan 1 , Ming-Hao Li 1 , Fa-Li Zhang 1 , Han Wang 1 , Kai-Yu Shen 1 , Ying Liu 1 , Yu-Jiang Sun 4 , Wei Shen 1 , Lan Li 1
Affiliation  

Zearalenone (ZEN) is a secondary metabolite, which is mainly produced by Fusarium fungi and exists in various feeds and agricultural products. Recently, an increasing amount of data has shown that ZEN, as an estrogen-like hormone, can have harmful effects on the female reproductive system, especially on oogenesis and folliculogenesis. Breast milk is considered to be the ideal form of nutrition for infants; however, there are some records of contaminants in food, such as mycotoxins, which may be transferred from maternal blood to milk. In this study, we investigated the toxic effects of breast milk on folliculogenesis in offspring following maternal ZEN exposure. Our results showed that maternal ZEN exposure significantly inhibited the process of primordial follicle (PF) assembly and reduced the number of PFs in suckled offspring’s ovaries. In addition, RNA-seq analysis showed that RIG-I-like receptor (RLRs) signaling pathways were activated after exposed to ZEN, which increased the expression levels of DNA damage (γ-H2AX, RAD51, and PARP1) and apoptosis related protein (BAX/BCL2 and Caspase-3). Finally, ZEN exposure interfered with follicular development, as evidenced by the reduced percentages of oocyte maturation and embryonic development when the offspring grew to adolescence. It is worth noting that maternal ZEN exposure disrupted the tri-methylation levels of H3K4, H3K9, and H3K27 in the offspring’s oocytes. Our results indicated that maternal ZEN exposure affected ovarian development in offspring through the breast milk, which may be detrimental to their reproductive capability in adult life.



中文翻译:

孕妇玉米赤霉烯酮暴露影响了后代卵巢卵泡的形成和发育。

玉米赤霉烯酮(ZEN)是次生代谢产物,主要由镰刀菌属生产真菌,存在于各种饲料和农产品中。近来,越来越多的数据表明,ZEN作为一种类似雌激素的激素,可能对女性生殖系统,特别是卵子发生和卵泡生成有有害作用。母乳被认为是婴儿营养的理想形式。但是,有一些食物中的污染物记录,例如霉菌毒素,可能会从母体血液转移到牛奶中。在这项研究中,我们调查了母体ZEN暴露后母乳对后代卵泡形成的毒性作用。我们的结果表明,母亲ZEN暴露显着抑制了原始卵泡(PF)的组装过程,并减少了哺乳后代卵巢中PF的数量。此外,RNA-seq分析显示,暴露于ZEN后,RIG-I样受体(RLRs)信号通路被激活,从而增加了DNA损伤(γ-H2AX,RAD51和PARP1)和凋亡相关蛋白(BAX / BCL2)的表达水平。和Caspase-3)。最后,ZEN暴露会干扰卵泡发育,这一点可以通过后代到青春期卵母细胞成熟和胚胎发育的百分比降低来证明。值得注意的是,母体ZEN暴露破坏了后代卵母细胞中H3K4,H3K9和H3K27的三甲基化水平。我们的结果表明,母亲ZEN暴露会通过母乳影响后代的卵巢发育,这可能对其成年后的生殖能力有害。RAD51和PARP1)和凋亡相关蛋白(BAX / BCL2和Caspase-3)。最后,ZEN暴露会干扰卵泡发育,这一点可以通过后代到青春期卵母细胞成熟和胚胎发育的百分比降低来证明。值得注意的是,母体ZEN暴露破坏了后代卵母细胞中H3K4,H3K9和H3K27的三甲基化水平。我们的结果表明,母亲ZEN暴露会通过母乳影响后代的卵巢发育,这可能对其成年后的生殖能力有害。RAD51和PARP1)和凋亡相关蛋白(BAX / BCL2和Caspase-3)。最后,ZEN暴露会干扰卵泡发育,这一点可以通过后代到青春期卵母细胞成熟和胚胎发育的百分比降低来证明。值得注意的是,母体ZEN暴露破坏了后代卵母细胞中H3K4,H3K9和H3K27的三甲基化水平。我们的结果表明,母亲ZEN暴露会通过母乳影响后代的卵巢发育,这可能对其成年后的生殖能力有害。值得注意的是,母体ZEN暴露破坏了后代卵母细胞中H3K4,H3K9和H3K27的三甲基化水平。我们的结果表明,母亲ZEN暴露会通过母乳影响后代的卵巢发育,这可能对其成年后的生殖能力有害。值得注意的是,母体ZEN暴露破坏了后代卵母细胞中H3K4,H3K9和H3K27的三甲基化水平。我们的结果表明,母亲ZEN暴露会通过母乳影响后代的卵巢发育,这可能对其成年后的生殖能力有害。

更新日期:2021-05-17
down
wechat
bug