In the area of stem cell research, fusion of somatic cells into pluripotent cells such as mouse embryonic stem (ES) cells induces reprogramming of the somatic nucleus and can be used to study the effect of trans-acting factors from the pluripotent cell on the pluripotent state of somatic nucleus. As many other groups, we previously established a porcine pluripotent cell line at a low potential. Therefore, here, we performed experiments to investigate if the fusion with mouse ES cell could improve the pluripotent state of porcine pluripotent cell. Our data showed that resultant mouse–porcine interspecies fused cells are AP positive, and could be passaged up to 20 passages. Different degrees of increases in expression of porcine pluripotent genes proved that pig-origin gene network can be programmed by mouse ES. Further differentiation study also confirmed these fused cells’ potential to form three germ layers. However, unexpectedly, we found that chromosome loss and aberrant (especially in porcine chromosomes) is severe after the cell fusion, implying that interspecies cell fusion may be not suitable to study porcine pluripotency without additional supportive conditions for genome stabilization.

中文翻译：

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小鼠胚胎干细胞与猪多能细胞的种间细胞融合

在干细胞研究领域，将体细胞融合到小鼠胚胎干 (ES) 细胞等多能细胞中会诱导体细胞核的重编程，可用于研究多能细胞的反式作用因子对多能细胞的影响。体细胞核的状态。与许多其他组一样，我们之前建立了低电位的猪多能细胞系。因此，在这里，我们进行了实验以研究与小鼠ES细胞的融合是否可以改善猪多能细胞的多能状态。我们的数据显示，所得小鼠-猪种间融合细胞为 AP 阳性，最多可传代 20 代。猪多能基因表达的不同程度增加证明猪源基因网络可以由小鼠ES编程。进一步的分化研究也证实了这些融合细胞形成三个胚层的潜力。然而，出乎意料的是，我们发现细胞融合后染色体丢失和异常（特别是在猪染色体中）很严重，这意味着如果没有额外的基因组稳定支持条件，种间细胞融合可能不适合研究猪的多能性。