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Transcriptome profiles of pre-pubertal and adult in vitro matured ovine oocytes obtained from FSH-stimulated animals
Reproduction in Domestic Animals ( IF 1.7 ) Pub Date : 2021-05-15 , DOI: 10.1111/rda.13951
Chen Ying 1 , Wu Yangsheng 2, 3, 4 , Lin Jiapeng 2, 3, 4 , Wang Liqin 2, 3, 4 , Li Xiaolin 1 , Liu Mingjun 1, 2, 3, 4 , Huang Juncheng 2, 3, 4
Affiliation  

Pre-pubertal females produce more oocytes than adult individuals, but the ability of oocytes to undergo embryonic development and produce viable offspring is less in pre-pubertal animals than in adult animals. Oocyte quality is associated with abnormal gene expression. To date, the transcriptome profiles of 1-month-old lamb oocytes after in vitro maturation (IVM) are poorly reported. This study aimed to identify differentially expressed genes (DEGs) in lamb oocytes with varying competencies, affecting oocyte competence. Using RNA sequencing (RNA developmental -seq) technology, the transcriptome profiles of 1-month-old lamb oocytes after IVM were assessed, and oocytes from adult sheep large follicles were used as controls. We found 11 up-regulated and 39 down-regulated DEGs in lamb oocytes. Gene Ontology analysis of DEGs showed that molecular functions were clustered in catalytic and binding activities, while biological processes were clustered in metabolic process, cellular process, single-organism process and biological regulation. Kyoto Encyclopedia of Genes and Genomes analysis showed that the DEGs were involved in oxidative phosphorylation, transforming growth factor-beta signalling pathway, or DNA replication. In lamb MII oocytes, down-regulation of oxidative phosphorylation genes (ATP5E, NDUFA7 and COX6C), thiol protease inhibitor (CSTB) and 26S proteasome component (SHFM1) and up-regulation of CUL1, MARCH7 and TRIM17 might cause low competence of lamb embryos. In conclusion, this study provided detailed information on mRNA transcriptomes in lamb oocytes after IVM, which offers insights into the reduced developmental potential of lamb oocytes.

中文翻译:

从 FSH 刺激动物获得的青春期前和成年体外成熟绵羊卵母细胞的转录组谱

青春期前的雌性比成年个体产生更多的卵母细胞,但在青春期前的动物中,卵母细胞经历胚胎发育并产生可存活后代的能力低于成年动物。卵母细胞质量与异常基因表达有关。迄今为止,体外成熟 (IVM) 后 1 个月大的羔羊卵母细胞的转录组图谱鲜有报道。本研究旨在鉴定具有不同能力的羔羊卵母细胞中影响卵母细胞能力的差异表达基因(DEG)。使用RNA测序(RNA发育-seq)技术,评估IVM后1个月大羔羊卵母细胞的转录组谱,并以成年绵羊大卵泡的卵母细胞作为对照。我们在羔羊卵母细胞中发现了 11 个上调和 39 个下调的 DEG。DEGs的基因本体分析表明,分子功能集中在催化和结合活动,而生物过程集中在代谢过程、细胞过程、单一生物过程和生物调控。京都基因和基因组百科全书分析表明,DEG 参与氧化磷酸化、转化生长因子-β 信号通路或 DNA 复制。在羔羊 MII 卵母细胞中,氧化磷酸化基因的下调(转化生长因子-β 信号通路或 DNA 复制。在羔羊 MII 卵母细胞中,氧化磷酸化基因的下调(转化生长因子-β 信号通路或 DNA 复制。在羔羊 MII 卵母细胞中,氧化磷酸化基因的下调(ATP5ENDUFA7COX6C )、硫醇蛋白酶抑制剂 ( CSTB ) 和 26S 蛋白酶体成分 ( SHFM1 ) 以及CUL1MARCH7TRIM17 的上调可能导致羔羊胚胎的低能力。总之,这项研究提供了有关 IVM 后羊卵母细胞 mRNA 转录组的详细信息,这提供了对羊卵母细胞发育潜力降低的见解。
更新日期:2021-05-15
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