Milk oligosaccharides are the prebiotic gold standard for neonates. This work investigated the concentration and profile of bovine milk oligosaccharides (BMO) in colostrum and other types of liquid feeds. Sialylated BMO displayed a broad range of concentrations (from nearly 1.7 g L⁻¹ to 100 mg L⁻¹) with 3′-sialyllactose, 6′-sialyllactose, and 6′-sialyl-N-acetyllactosamine having the highest concentration in the 1st milking postpartum but decreasing rapidly until reaching a stable concentration at the 3rd–5th milking. While 3′-sialyllactose was present at the highest concentration in all samples, 6′-sialyl-N-acetyllactosamine concentration was higher than 6′-sialyllactose in the 1st milking but lower than 6′-sialyllactose in the 3rd–5th milking. BMO abundance in colostrum was 7–9 fold higher than in other liquid feeds (saleable, non-saleable, and saleable mixed with non-saleable milk). Sialylated BMO were the predominant type in colostrum, whereas neutral non-fucosylated were similar in all feeds. Five fucosylated BMO were also identified.

牛奶低聚糖是新生儿益生元的金标准。这项工作调查了初乳和其他类型液体饲料中牛乳寡糖 (BMO) 的浓度和分布。唾液酸化的 BMO 显示出广泛的浓度范围（从近 1.7 g L ^-1到 100 mg L ^-1），其中 3'-唾液酸乳糖、6'-唾液酸乳糖和 6'-唾液酸-N-乙酰乳糖胺在第一产后挤奶，但迅速下降，直到在第 3-5 次挤奶时达到稳定浓度。虽然 3'-唾液酸乳糖在所有样品中的浓度最高，但 6'-唾液酸-N-乙酰乳糖胺浓度在第 1 次挤奶时高于 6'-唾液酸乳糖，但在第 3-5 次挤奶时低于 6'-唾液酸乳糖。初乳中的 BMO 丰度比其他液体饲料（可销售、不可销售和可销售与不可销售牛奶混合）高 7-9 倍。唾液酸化的 BMO 是初乳中的主要类型，而中性非岩藻糖基化的 BMO 在所有饲料中都相似。还鉴定了五种岩藻糖基化的 BMO。