Abstract

A number of polyphenol fractions were extracted from the brown alga Thalassiophyllum clathrus (Postels & Ruprecht, 1840) by the method of liquid–liquid extraction using solvents of increasing polarity, and their antioxidant activities were studied. The extract obtained with ethyl acetate was characterized by a maximum polyphenol content of 581.2 ± 8.4 mg/g equivalent to gallic acid and a maximum antioxidant activity (22.0 ± 0.1 μg/mL in EC₅₀ units (2,2-diphenyl-1-picrylhydrazyl) and 5.84 ± 1.64 μmol/g equivalent to Trolox). Six purified polyphenol fractions were isolated from the ethyl acetate extract by preparative size-exclusion chromatography; the antioxidant activity was preserved. The conditions for separation of the purified polyphenol fractions through reverse phase chromatography were selected as follows: the pH of elution buffer was 2.0–3.0; the water–acetonitrile gradient was supplemented with formic acid; the temperature was 25°C; and the flow rate was 1 mL/min. It has been shown that the extract from T. clathrus contains polyphenolic compounds of the following groups: phenolic acids (gallic acid), hydroxycinnamic acids (caffeic acid and its derivative, chlorogenic acid), and flavonols (kaempferol and quercetin).

中文翻译：

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褐藻藻类（Thalasiophyllum clathrus）的提取物中的多酚馏分（Postels＆Ruprecht，1840）及其抗氧化活性的研究

摘要

通过使用增加极性的溶剂进行液-液萃取方法，从褐藻藻类（Thalasiophyllum clathrus）（Postels＆Ruprecht，1840）中提取了许多多酚级分，并研究了它们的抗氧化活性。用乙酸乙酯获得的提取物的特征在于最大的多酚含量为581.2±8.4 mg / g（相当于没食子酸）和最大的抗氧化活性（在EC _50中为22.0±0.1μg/ mL单位（2,2-二苯基-1-吡啶并肼基）和5.84±1.64μmol/ g等效于Trolox）。通过制备尺寸排阻色谱法从乙酸乙酯提取物中分离出六种纯化的多酚级分。抗氧化活性得以保留。通过反相色谱法分离纯化的多酚馏分的条件选择如下：洗脱缓冲液的pH为2.0-3.0。在水-乙腈梯度中补充甲酸；温度为25℃。流速为1 mL / min。已经表明，来自T. clathrus的提取物包含以下多酚化合物：酚酸（没食子酸），羟基肉桂酸（咖啡酸及其衍生物，绿原酸）和黄酮醇（山ka酚和槲皮素）。