Photoacoustic microscopy (PAM) is an important imaging tool that can noninvasively visualize the anatomical structure of living animals. However, the limited scanning area restricts traditional PAM systems for scanning a large animal. Here, we firstly report a dual-channel PAM system based on a custom-made slider-crank scanner. This novel scanner allows us to stably capture an ultra-widefield scanning area of 24 mm at a high B-scan speed of 32 Hz while maintaining a high signal-to-noise ratio. Our system's spatial resolution is measured at ∼3.4 μm and ∼37 μm for lateral and axial resolution, respectively. Without any contrast agent, a dragonfly wing, a nude mouse ear, an entire rat ear, and a portion of mouse sagittal are successfully imaged. Furthermore, for hemodynamic monitoring, the mimicking circulating tumor cells using magnetic contrast agent is rapidly captured in vitro. The experimental results demonstrated that our device is a promising tool for biological applications.

光声显微镜 (PAM) 是一种重要的成像工具，可以无创地可视化活体动物的解剖结构。然而，有限的扫描区域限制了传统 PAM 系统扫描大型动物的能力。在这里，我们首先报告了一个基于定制滑块曲柄扫描仪的双通道 PAM 系统。这种新型扫描仪使我们能够以 32 Hz 的高 B 扫描速度稳定捕获 24 mm 的超宽场扫描区域，同时保持高信噪比。我们系统的空间分辨率分别为~3.4 μm 和~37 μm 的横向和轴向分辨率。在没有任何造影剂的情况下，蜻蜓翅膀、裸鼠耳朵、整个老鼠耳朵和部分老鼠矢状面都被成功成像。此外，对于血流动力学监测，体外。实验结果表明，我们的设备是一种很有前途的生物应用工具。