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Mangrove horseshoe crab (Carcinoscorpius rotundicauda Latreille, 1802) populations reveal genetic break in Strait of Malacca, with connectivity along southern coasts of Peninsular Malaysia
Aquatic Conservation: Marine and Freshwater Ecosystems ( IF 2.4 ) Pub Date : 2021-05-14 , DOI: 10.1002/aqc.3552
Nur Fairuz‐Fozi 1 , Ludwig Triest 2 , Nurul Ashikin Mat Zauki 1 , Anne Marie Kaben 1 , Bryan Raveen Nelson 3 , Anil Chatterji 4 , Mohd Fadzil Akhir 1 , Behara Satyanarayana 1, 5 , Farid Dahdouh‐Guebas 2, 5
Affiliation  

  1. The mangrove horseshoe crab, Carcinoscorpius rotundicauda, has divergent populations between the east and west coasts of Peninsular Malaysia, with the southern coast acting as a land barrier. The actual position of such a genetic break along Peninsular Malaysia as well as the connectivity status of the southernmost C. rotundicauda populations with east and west coast populations remain unexplored, however.
  2. The aim was to investigate the genetic diversity and structure of C. rotundicauda populations from the west (Kuala Sepetang in State Perak), east (Balok in State Pahang), and southern (Pendas in State Johor) coasts of Peninsular Malaysia. Haemolymph samples from adult C. rotundicauda specimens (n = 152) and eggs from their freshly deposited nests (n = 190) were collected monthly (from January 2016 to January 2017) for the sequencing of mitochondrial cytochrome c oxidase subunit I (COI).
  3. Gene isolates of C. rotundicauda from the present study were compared with National Center for Biotechnology Information (NCBI) GenBank sequences to cover most of the range of the species in Asia. A neighbour-joining tree strongly supported two clades, separating the west-coast populations from the south- and east-coast populations, with further substructure patterns.
  4. Both haplotype network and barrier analyses revealed a genetic break within the Strait of Malacca instead of the southern tip of Peninsular Malaysia. The southernmost samples from the Strait of Johor formed a haplotypic diverse gene pool that appeared only as a subclade of the eastern populations. In a detailed haplotype network of 347 individuals, individuals with similar COI sequences indicate connectivity between C. rotundicauda on the east and C. rotundicauda on south and south west of Peninsular Malaysia.
  5. Overall, the genetic break between C. rotundicauda populations is better explained by the convergent ocean currents and available mangrove habitats on the west coast (i.e. Strait of Malacca), rather than the point of the Malay Peninsula acting as a land barrier alone.


中文翻译:

红树林马蹄蟹 (Carcinoscorpius rotundicauda Latreille, 1802) 种群揭示了马六甲海峡的基因断裂,与马来西亚半岛南部海岸的连通性

  1. 红树林马蹄蟹Carcinoscorpius rotundicauda在马来西亚半岛东海岸和西海岸之间有不同的种群,南部海岸充当陆地屏障。然而,这种沿马来西亚半岛的遗传断裂的实际位置以及最南端的圆尾尾目种群与东海岸和西海岸种群的连接状态仍未得到探索。
  2. 目的是调查来自马来西亚半岛西部(霹雳州的 Kuala Sepetang)、东部(彭亨州的 Balok)和南部(柔佛州的 Pendas)海岸的圆尾尾目鱼种群的遗传多样性和结构。 每月(从 2016 年 1 月至 2017 年 1 月)收集来自成年尾蚴标本(n  = 152)和来自其新鲜巢穴的卵(n = 190)的血淋巴样本,用于线粒体细胞色素c氧化酶亚基 I(COI)的测序 。
  3. 将本研究中圆尾蚴的基因分离物与国家生物技术信息中心 (NCBI) 的基因库序列进行比较,以覆盖亚洲大部分物种范围。一棵相邻的树强烈支持两个进化枝,将西海岸种群与南海岸和东海岸种群分开,并具有进一步的子结构模式。
  4. 单倍型网络和屏障分析都揭示了马六甲海峡内的基因断裂,而不是马来西亚半岛的南端。柔佛海峡最南端的样本形成了一个单倍型多样的基因库,仅作为东部种群的一个分支出现。在347个人的详细单倍型网络,具有相似的个体COI序列表明之间的连接C.圆尾东和C.圆尾在南部和马来西亚半岛的西南部。
  5. 总体而言,C. rotundicauda种群之间的遗传断裂可以更好地解释为会聚洋流和西海岸(即马六甲海峡)上可用的红树林栖息地,而不是马来半岛的这一点单独作为陆地屏障。
更新日期:2021-07-18
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