Genome sequence-driven molecular typing tools have the potential to uncover the population biology and genetic diversity of rapidly evolving plant pathogens like Magnaporthe oryzae. Here, we report a new molecular typing technique -a digitally portable tool for population genetic analysis of M. oryzae to decipher the genetic diversity. Our genotyping tool exploiting allelic variations in housekeeping and virulence genes coupled with pathotyping revealed a prevalence of genetically homogenous populations within a single-field and plant niches such as leaf and panicle. The M. oryzae inciting leaf-blast and panicle-blast were confirmed to be genetically identical with no or minor nucleotide polymorphism in 17 genomic loci analyzed. Genetic loci such as Mlc1, Mpg1, Mps1, Slp1, Cal, Ef-Tu, Pfk, and Pgk were highly polymorphic as indicated by the haplotype-diversity, the number of polymorphic sites, and the number of mutations. The genetically homogenous single field population showed high virulence variability or diversity on monogenic rice differentials. The study indicated that the genetic similarity displayed by the isolates collected from a particular geographical location had no consequence on their virulence pattern on rice differentials carrying single/multiple resistance genes. The data on virulence diversity showed by the identical Sequence Types (STs) is indicative of no congruence between polymorphic virulence genes-based pathotyping and conserved housekeeping genes-based genotyping.

基因组序列驱动的分子分型工具有可能揭示快速进化的植物病原体（如稻瘟病菌）的种群生物学和遗传多样性。在这里，我们报告了一种新的分子分型技术 - 一种用于米曲霉种群遗传分析以破译遗传多样性的数字便携式工具。我们的基因分型工具利用看家和毒力基因的等位基因变异与病理分型相结合，揭示了单田和植物生态位（如叶和穗）中遗传同质种群的普遍存在。的稻瘟病菌煽动叶-BLAST和穗鼓风被证实为无或轻微核苷酸多态性遗传上相同的17个基因座进行分析。遗传位点，如Mlc1、Mpg1、Mps1、Slp1、Cal、Ef-Tu、Pfk和Pgk是高度多态的，如单倍型多样性、多态位点数量和突变数量所示。遗传同质的单田种群在单基因水稻差异化上表现出高毒力变异性或多样性。该研究表明，从特定地理位置收集的分离株所显示的遗传相似性对其对携带单/多抗性基因的水稻差异的毒力模式没有影响。相同序列类型 (ST) 显示的毒力多样性数据表明基于多态毒力基因的病理分型和基于保守看家基因的基因分型之间没有一致性。