ABSTRACT

1. The objective of this study was to identify the effects of the antioxidant alpha-tocopherol acetate (ATA) and alpha lipoic acid (ALA) which have anti-inflammatory effects on developmental onset, severity and the progression of wooden breast (WB) based on Pectoralis major (P. major) muscle morphology and expression of genes associated with WB during the first three weeks post-hatch.

2. A total of 160 newly hatched Ross 708 broiler chicks were randomly assigned in a replicated trial to either a control group or three dietary treatments (ATA 160 mg/kg feed, ALA 500 mg/kg feed or in combination).

3. Microscopic changes associated with WB began at one week of age in all groups. The ATA acetate and ALA fed in combination decreased WB severity at two weeks of age (P = 0.05) and ATA alone or in combination reduced severity at three weeks of age compared to the control group (P = 0.05). Expression of myogenic determination factor 1 and peroxisome proliferator-activated receptor gamma was reduced in all dietary treatments compared to the control at three weeks of age (P ≤ 0.05), which suggested reduced muscle degeneration and lipid deposition.

4. ATA and ALA fed both independently and in combination had a positive effect on mitigating WB severity microscopically as early as two weeks of age.

摘要

1. 本研究的目的是确定抗氧化剂 α-生育酚乙酸酯 (ATA) 和α-硫辛酸 (ALA) 对发育开始、严重程度和木制乳房 (WB) 进展具有抗炎作用上胸大肌（P.主要）肌肉形态和与WB相关的基因表达的前三个星期后孵化期间。

2. 在重复试验中，将总共 160 只新孵化的 Ross 708 肉鸡随机分配到对照组或三种日粮处理组（ATA 160 mg/kg 饲料、ALA 500 mg/kg 饲料或组合）。

3. 与 WB 相关的微观变化开始于所有组的 1 周龄。与对照组相比，ATA 醋酸盐和 ALA 联合喂养降低了两周大的 WB 严重程度（P = 0.05），而单独或联合喂养的 ATA 降低了三周大时的严重程度（P = 0.05）。与三周龄的对照组相比，所有饮食处理中生肌决定因子 1 和过氧化物酶体增殖物激活受体 γ 的表达均降低（P ≤ 0.05），这表明肌肉退化和脂质沉积减少。

4. ATA 和 ALA 单独和联合喂养早在两周大时就在显微镜下对减轻 WB 严重程度产生积极影响。