This study aimed to evaluate the potential use of some tree leaves including almond (Prunus amygdalus L.), pomegranate (Punica granatum L.), apple (Malus domestica Borkh.), pistachio (Pistacia vera L.), and walnut (Juglans regia L.) compared to whole plant alfalfa (Medicago sativa L.) in sheep feeding through in vitro experiments in order to screening of the resources and then selecting the most suitable candidate resource for in vivo experiment. Chemical and mineral composition, in vitro gas production, and fermentation parameters of leaves were also determined. The results indicated that the crude protein (CP) ranged from 12.12 % for almond leaves (AL) to 16.77 % for alfalfa, on dry matter (DM) basis. The highest and lowest neutral detergent fiber (NDF) content was observed in alfalfa (48.50 %) and pomegranate leaves (26.45 %), respectively (P < 0.0001). The range of metabolisable energy (ME) differs from 6.76 for pomegranate leaves to 10.50 MJ/kg DM for AL. Alfalfa and AL exhibited the lowest (9.53 g kg DM) and the highest calcium (44.15 g/kg DM) concentration, respectively (P < 0.0001). The highest true organic matter (TOMD) and dry matter digestibility (TDMD) (88.44 and 87.88 %, respectively) was also obtained in AL (P < 0.0001). It was found that AL had the highest potential gas production, and total volatile fatty acids (VFA) values of 61.05 ml/200 mg DM, and 48.33 mmol/L, respectively (P < 0.0001). In in vivo experiment, the effects of partial substitution of dietary forage with AL at 0, 5.8, 11.5, and 23 % of diet DM on growth performance, nutrient digestibility, ruminal fermentation, and blood metabolites were investigated using thirty-two Baluchi male lambs [28 ± 1.5 kg body weight (BW)] in a completely randomized design for 90 days. Compared to the control group, dry matter intake (DMI, P = 0.02), average daily gain (ADG, P = 0.05), DM (P = 0.0004), organic matter (OM, P = 0.003) and NDF digestibility (P = 0.0005), total VFA (P = 0.02), and plasma total antioxidant capacity (TAC, P = 0.0005) increased when high level of almond (HAL) was added to the diet. Overall, it is revealed that AL had a high nutritional value and could be used up to 23 % of diet DM in the diet of Baluchi male lambs as an inexpensive alternative forage without negative effects on performance and blood metabolites.

这项研究旨在评估某些树叶的潜在用途，包括杏仁（Prunus amygdalus L.），石榴（Punica granatum L.），苹果（Malus domestica Borkh。），开心果（Pistacia vera L.）和核桃（Juglans regia） L.）与全草苜蓿（Medicago sativa L.）在绵羊中通过体外实验饲喂相比，以便筛选资源，然后选择最合适的体内实验候选资源。化学和矿物成分，体外还确定了产气量和叶片的发酵参数。结果表明，基于干物质（DM），粗蛋白（CP）的范围从杏仁叶（AL）的12.12％到苜蓿的16.77％。在苜蓿（48.50％）和石榴叶（26.45％）中分别观察到最高和最低的中性洗涤剂纤维（NDF）含量（P <0.0001）。代谢能（ME）的范围从石榴叶的6.76到AL的10.50 MJ / kg DM有所不同。苜蓿和AL分别显示最低（9.53 g / kg DM）和最高钙（44.15 g / kg DM）浓度（P <0.0001）。在AL也获得了最高的真有机物（TOMD）和（分别88.44 87.88和％），干物质消化率（TDMD）（P<0.0001）。发现AL具有最高的潜在产气量，总挥发性脂肪酸（VFA）值分别为61.05 ml / 200 mg DM和48.33 mmol / L（P <0.0001）。在体内实验中，使用32头Bal路支公羊羔研究了日粮DM在0、5.8、11.5和23％的日粮DM中部分替代日粮饲草对生长性能，营养物质消化率，瘤胃发酵和血液代谢产物的影响。 [28±1.5 kg体重（BW）]，完全随机设计，可使用90天。与对照组相比，干物质摄入量（DMI，P = 0.02），平均日增重（ADG，P = 0.05），DM（P = 0.0004），有机质（OM，P当在日粮中添加高含量杏仁（HAL）时，NDF消化率（P = 0.0005），总VFA（P = 0.02）和血浆总抗氧化能力（TAC，P = 0.0005）会增加。总的来说，它表明AL具有很高的营养价值，在Bal路支雄性羔羊的日粮中，AL的日粮DM用量高达23％，可以作为廉价的替代草料，而对性能和血液代谢产物没有负面影响。