Our previous research showed that increased phosphorylation of connexin (Cx)36 indicated extended coupling of AII amacrine cells (ACs) in the rod-dominant mouse myopic retina. This research will determine whether phosphorylation at serine 276 of Cx35-containing gap junctions increased in the myopic chicken, whose retina is cone-dominant. Refractive errors and ocular biometric dimensions of 7-days-old chickens were determined following 12 h and 7 days induction of myopia by a −10D lens. The expression pattern and size of Cx35-positive plaques were examined in the early (12 h) and compensated stages (7 days) of lens-induced myopia (LIM). At the same time, phosphorylation at serine 276 (functional assay) of Cx35 in strata 5 (S5) of the inner plexiform layer was investigated. The axial length of the 7 days LIM eyes was significantly longer than that of non-LIM controls (P < 0.05). Anti-phospho-Ser276 (Ser276-P)-labeled plaques were significantly increased in LIM retinas at both 12 h and 7 days. The density of Ser276-P of Cx35 was observed to increase after 12 h LIM. In the meanwhile, the areas of existing Cx35 plaques did not change. As there was more phosphorylation of connexin35 at Ser276 at both the early and late stages (12 h) and 7 days of LIM chicken retinal activity, the coupling with ACs could be increased in myopia development of the cone-dominated chicken retina.

中文翻译：

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近视鸡视网膜中功能性连接蛋白35增加

我们之前的研究表明，连接蛋白 (Cx) 36 的磷酸化增加表明杆状显性小鼠近视视网膜中 AII 无长突细胞 (ACs) 的耦合延长。这项研究将确定在视网膜以视锥细胞为主的近视鸡中，含 Cx35 的间隙连接的丝氨酸 276 处的磷酸化是否增加。在用-10D 镜片诱导近视 12 小时和 7 天后测定 7 日龄鸡的屈光不正和眼部生物特征尺寸。在晶状体诱导近视 (LIM) 的早期 (12 h) 和补偿阶段 (7 天) 检查 Cx35 阳性斑块的表达模式和大小。同时，研究了内丛状层第5层（S5）中Cx35的丝氨酸276（功能测定）的磷酸化。磷< 0.05)。在 12 小时和 7 天，LIM 视网膜中抗磷酸化 Ser276 (Ser276-P) 标记的斑块显着增加。观察到 Cx35 的 Ser276-P 的密度在 12 小时 LIM 后增加。同时，现有Cx35斑块的面积没有改变。由于在 LIM 鸡视网膜活动的早期和晚期（12 小时）和 7 天，Ser276 处的连接蛋白 35 磷酸化更多，因此在以视锥细胞为主的鸡视网膜的近视发展中，与 AC 的耦合可能会增加。