This article aims to envisage future perspectives of the lymphocyte transformation test (LTT). We describe the select innovative techniques, which can be integrated at different stages of the LTT to potentially improve the sensitivity, specificity, or practicability of the LTT. We first focus upon the cell sorting techniques comprising immunomagnetic cell separation and flow cytometry, which can be implemented prior and after the LTT culturing step to concentrate and quantify specific immune cell types. Further, we elaborate upon three important omics techniques such as transcriptomics, proteomics, and metabolomics, which can be integrated downstream of the LTT to analyze molecular changes in specific immune cells following drug induced activation and proliferation. We also develop visions, how state of the art techniques used in other scientific fields, can be transferred and applied in the context of in-vitro detection of drug allergy.

本文旨在展望淋巴细胞转化试验 (LTT) 的未来前景。我们描述了精选的创新技术，这些技术可以在 LTT 的不同阶段进行整合，以潜在地提高 LTT 的灵敏度、特异性或实用性。我们首先关注细胞分选技术，包括免疫磁性细胞分离和流式细胞术，这些技术可以在 LTT 培养步骤之前和之后实施，以浓缩和量化特定的免疫细胞类型。此外，我们详细阐述了三种重要的组学技术，如转录组学、蛋白质组学和代谢组学，它们可以整合到 LTT 的下游，以分析药物诱导激活和增殖后特定免疫细胞的分子变化。我们也发展愿景，在其他科学领域中使用的最先进技术如何在体外检测药物过敏的情况下转移和应用。