Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.

中文翻译：

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使用超分子宿主增强基于小分子的蛋白质标签荧光的步骤

用基于小分子的荧光探针标记蛋白质为确定蛋白质在活细胞中的表达水平和定位提供了强大的工具。我们提出了一种使用超分子宿主（例如β-环糊精（β-CD））来增加和稳定此类探针发射的方法。尽管在存在β-CD的情况下，His-tag结合探针的发射增强，但是探针-β-CD复合物与His-tagged蛋白的结合导致部分β-CD移位，因此，发射减少信号。