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Two internal bottlenecks cause the overflow metabolism leading to poly(3-hydroxybutyrate) production in Azohydromonas lata DSM1123.
Journal of Environmental Chemical Engineering ( IF 7.7 ) Pub Date : 2021-05-14 , DOI: 10.1016/j.jece.2021.105665
Felipe Scott , Luz Yañez , Raúl Conejeros , Blanca Araya , Alberto Vergara-Fernández

Polyhydroxybutyrate production in the bacteria Azohydromonas lata DSM1123 has been regarded as growth-associated based on batch experiments. However, growth association can only be confirmed under chemostat culture. In this work, an experimental approach followed by Flux Balance Analysis (FBA) modeling was used to analyze the behavior of this strain under glucose, oxygen, and nitrogen-limited conditions. The model allowed the identification of candidate enzymes constraining the flux through the tricarboxylic acid cycle, and the role of ATP synthase as the key flux controlling enzyme for the respiratory metabolism, in terms of its share of the total metabolically active protein. The results presented in this work and the model developed could assist in the economic and environmental assessment of new continuous processes to produce PHB and other metabolites in A. lata DSM1123, a strain capable of hydrogen use and CO2 fixation.



中文翻译:

两个内部瓶颈导致溢流代谢,从而导致Azohydromonas lata DSM1123中产生聚(3-羟基丁酸酯)。

细菌Azohydromonas lata中的多羟基丁酸酯生产根据批次实验,DSM1123被视为与生长相关。但是,仅在恒化器培养下才能确认生长缔合。在这项工作中,采用实验方法和通量平衡分析(FBA)建模方法来分析该菌株在葡萄糖,氧气和氮限制条件下的行为。该模型允许鉴定约束三羧酸循环通量的候选酶,以及就其在总代谢活性蛋白中所占的份额而言,ATP合酶作为呼吸代谢关键通量控制酶的作用。这项工作中提出的结果和开发的模型可以帮助经济和环境评估新的连续过程以生产A. lata中的PHB和其他代谢物DSM1123,能够使用氢气和固定CO 2的菌株。

更新日期:2021-05-14
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