Rhizosphere ( IF 3.7 ) Pub Date : 2021-05-13 , DOI: 10.1016/j.rhisph.2021.100377 David Pessanha Siqueira , Andréia Francisca Riter Netto , Juliana Müller Freire , Deborah Guerra Barroso
Forest association with arbuscular mycorrhizal fungi (AMF) can improve the uptake of nutrients and hence the growth of host plants and it exerts a crucial role in tropical nutrient-poor soils. Our work aimed to verify if there is natural mycorrhizal colonization in Plathymenia reticulata and Dalbergia nigra monospecific plantations. Soil sampling was collected in the vicinity of the trees and fine roots were selected. The fine roots were washed, clarified and then stained. The spore isolation and identification were assessed by the wet sieving and sucrose density centrifugation. Both P. reticulata and D. nigra roots have fungal structures (vesicles and hyphae). The species Glomus macrocarpum and Ambispora leptoticha were identified in the soil under P. reticulata and Glomus macrocarpum, Glomus agregatum, and Claroideoglomus etunicatum in the soil under D. nigra. Both species can naturally be associated with AMF and we can suggest that different fungi are colonizing the roots of each species.
中文翻译:
巴西东南部两个热带固氮单种人工林的天然菌根协会
森林与丛枝菌根真菌(AMF)的结合可以提高养分的吸收,从而改善寄主植物的生长,并且在热带贫瘠的土壤中起着至关重要的作用。我们的工作旨在验证网斑疟原虫和黑檀中的单种人工林是否存在天然的菌根定植。在树木附近收集土壤样品,并选择细根。将细根清洗,澄清然后染色。通过湿筛和蔗糖密度离心法评估孢子的分离和鉴定。既P.网状和D.黑根具有真菌结构(小泡和菌丝)。物种Glomus macrocarpum和Ambispora leptoticha在土壤中被确定下P.马丁和球囊大果,球囊agregatum和Claroideoglomus etunicatum下土壤D.黑。这两个物种自然都可以与AMF相关联,我们可以建议在每个物种的根部定植不同的真菌。