The aim of the present study is to evaluate the antioxidant activity (AA) of different fractions of the supercritical extract of Baccharis uncinella, in addition to investigating and relating AA to the potential antitumor action in bladder cancer (T24) and glioblastoma cells (U87) and also its toxicity against normal cells (Vero) for the fraction with the highest percentage of free radical scavenging (% FRS). The extracts were obtained sequentially by extraction with supercritical CO₂ (150 and 200 bar and 60 °C) using ethanol as a co-solvent. The fractionation was carried out by column chromatography with hexane, dichloromethane, ethyl acetate and methanol as eluents. AA was tested by the DPPH free radical scavenging method and cell toxicity tests were performed by the MTT assay. The methanolic fraction had the highest AA, with %FRS of 86.7 %, very close to the antioxidant standard used in the test, quercetin, with 87.9 %. The methanolic fraction was able to reduce cell viability to less than 50 % at a concentration of 158.8 μg/mL to T24, 213.9 μg/mL to U87 and 702.5 μg/mL to Vero cells. According to the results, the methanolic fraction of the supercritical extract of B. uncinella has an antiproliferative effect for the tumor cells tested, with less effects on the normal cells evaluated.

本研究的目的是评估Baccharis uncinella超临界提取物不同部分的抗氧化活性（AA），此外还研究了AA与膀胱癌（T24）和胶质母细胞瘤细胞（U87）的潜在抗肿瘤作用并将其联系起来。以及对自由基清除率最高（％FRS）的馏分对正常细胞（Vero）的毒性。通过用超临界CO ₂萃取来依次获得萃取物（150 and 200 bar and 60°C），使用乙醇作为助溶剂。通过柱色谱法使用己烷，二氯甲烷，乙酸乙酯和甲醇作为洗脱剂进行分馏。通过DPPH自由基清除方法测试AA，通过MTT分析进行细胞毒性测试。甲醇级分的AA最高，％FRS为86.7％，非常接近测试中使用的抗氧化剂标准槲皮素，为87.9％。甲醇级分能够将细胞活力降低到小于50％，其中T24浓度为158.8μg/ mL，U87浓度为213.9μg/ mL，Vero细胞浓度为702.5μg/ mL。根据结果​​，Uncinella的超临界提取物的甲醇级分对所测试的肿瘤细胞具有抗增殖作用，而对所评估的正常细胞的影响则较小。