Measurements of the dynamic surface elasticity and surface pressure of solutions of green fluorescent protein (GFP) indicate the formation of a dense protein monolayer with a relatively high surface elasticity of approximately 65 mN/m, and are typical for solutions of globular proteins. The mixed solutions of GFP and poly(N,N-diallyl-N-hexyl-N-methylammonium chloride) (PDAHMAC) demonstrate more complex behavior. During the first adsorption step the surface properties are determined by an unbound component, while the next step is mainly the adsorption of the complexes. The dynamic surface elasticity at relatively low polyelectrolyte concentrations (< 2*10⁻⁴ g/l) is close to the values for pure protein solutions when the surface pressure is less than 15 mN/m. At higher surface pressures the adsorption of complexes results in an increase of the surface elasticity up to 80 mN/m. If PDAHMAC concentrations are higher than 2*10⁻⁴ g/l, the kinetic dependences of the dynamic surface elasticity become non-monotonic and this quantity does not exceed 45 mN/m. The local maximum of the surface elasticity is a result of the formation of the distal region of the surface layer by the polyelectrolyte. Beyond the subsequent local minimum the surface elasticity starts to increase again due to the adsorption of GFP/ PDAHMAC complexes.

绿色荧光蛋白 (GFP) 溶液的动态表面弹性和表面压力的测量表明形成了致密的蛋白质单层，表面弹性相对较高，约为 65 mN/m，并且是球状蛋白质溶液的典型特征。GFP 和聚 (N,N-二烯丙基-N-己基-N-甲基氯化铵) (PDAHMAC) 的混合溶液表现出更复杂的行为。在第一个吸附步骤中，表面性质由未结合的组分决定，而下一步主要是复合物的吸附。在相对低的聚电解质浓度 (< 2*10 ^-4当表面压力小于 15 mN/m 时，g/l) 接近于纯蛋白质溶液的值。在更高的表面压力下，复合物的吸附导致表面弹性增加至 80 mN/m。如果 PDAHMAC 浓度高于 2*10 ^-4 g/l，则动态表面弹性的动力学依赖性变得非单调并且该量不超过 45 mN/m。表面弹性的局部最大值是由聚电解质形成表面层的远端区域的结果。由于 GFP/PDAHMAC 复合物的吸附，超过随后的局部最小值，表面弹性开始再次增加。