Background

Cold stress is the main abiotic stress in rice, which seriously affects the growth and yield of rice. Identification of cold tolerance genes is of great significance for rice to solve these problems. GATA-family transcription factors involve diverse biological functions, however, their role in cold tolerance in rice remains unclear.

Results

In this study, a GATA-type zinc finger transcription factor OsGATA16, which can improve cold tolerance, was isolated and characterized from rice. OsGATA16 belongs to OsGATA subfamily-II and contains 11 putative phosphorylation sites, a nuclear localization signal (NLS), and other several conserved domains. OsGATA16 was expressed in all plant tissues, with the strongest in panicles. It was induced by cold and ABA treatments, but was repressed by drought, cytokinin and JA, and acted as a transcriptional suppressor in the nucleus. Overexpression of OsGATA16 improves cold tolerance of rice at seedling stage. Under cold stress treatments, the transcription of four cold-related genes OsWRKY45–1, OsSRFP1, OsCYL4, and OsMYB30 was repressed in OsGATA16-overexpressing (OE) rice compared with wild-type (WT). Interestingly, OsGATA16 bound to the promoter of OsWRKY45–1 and repressed its expression. In addition, haplotype analysis showed that OsGATA16 polarized between the two major rice subspecies japonica and indica, and had a non-synonymous SNP8 (336^G) associated with cold tolerance.

Conclusion

OsGATA16 is a GATA transcription factor, which improves cold tolerance at seedling stage in rice. It acts as a positive regulator of cold tolerance by repressing some cold-related genes such as OsWRKY45–1, OsSRFP1, OsCYL4 and OsMYB30. Additionally, OsGATA16 has a non-synonymous SNP8 (336^G) associated with cold tolerance on CDS region. This study provides a theoretical basis for elucidating the mechanism of cold tolerance in rice and new germplasm resources for rice breeding.

中文翻译：

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OsGATA16 是一种 GATA 转录因子，通过在水稻幼苗期抑制 OsWRKY45-1 来赋予耐寒性

背景

冷胁迫是水稻主要的非生物胁迫，严重影响水稻的生长和产量。鉴定耐寒基因对水稻解决这些问题具有重要意义。GATA家族转录因子涉及多种生物学功能，然而，它们在水稻耐寒性中的作用仍不清楚。

结果

本研究从水稻中分离并鉴定了一种能提高耐寒性的GATA型锌指转录因子OsGATA16 。OsGATA16 属于 OsGATA 亚家族-II，包含 11 个推定的磷酸化位点、一个核定位信号 (NLS) 和其他几个保守结构域。OsGATA16在所有植物组织中均有表达，在圆锥花序中表达最强。它由冷和 ABA 处理诱导，但被干旱、细胞分裂素和 JA 抑制，并在细胞核中充当转录抑制因子。OsGATA16的过表达提高水稻苗期耐寒性。在冷胁迫下，四个冷相关基因OsWRKY45-1、OsSRFP1、与野生型 (WT) 相比，OsGATA16 过表达 (OE) 水稻中的 OsCYL4和OsMYB30受到抑制。有趣的是，OsGATA16 与 OsWRKY45-1 的启动子结合并抑制其表达。此外，单倍型分析表明OsGATA16在两个主要水稻亚种粳稻和籼稻之间极化，并具有与耐寒性相关的非同义SNP8（336 ^{G ）。}

结论

OsGATA16是一种GATA转录因子，可提高水稻幼苗期的耐寒性。它通过抑制一些与寒冷相关的基因（如OsWRKY45-1、OsSRFP1、OsCYL4和OsMYB30 ）作为耐寒性的正调节剂。此外，OsGATA16 具有与 CDS 区域的耐寒性相关的非同义 SNP8 (336 ^{G )。}本研究为阐明水稻耐寒机制及水稻育种新种质资源提供理论依据。