The biochemical reactions of aerobic microbial respiration (AMR) suggest that silage temperature (T_si) rise, oxygen (O₂) consumption and carbon dioxide (CO₂) emission may be equally useful as indicators of silage deterioration during feed-out, but only temperature has been used extensively to assess aerobic stability. Here we extend the study of aerobic stability to incorporate AMR of silage by developing a novel experimental cell integrated with multiple sensors. Silage samples, ensiled from a triticale crop, were made in twelve air-tight barrels (60 L), packed to bulk densities of 190 or 250 kg m⁻³ dry matter (DM). T_si and O₂ measurements were co-located at 15- and 30-cm behind the working face. CO₂ was measured as flux across the working face. The experimental period of aerobic exposure was 7 days. We provide the first reports of: (i) distinct aerobic responses of these parameters, showing that T_si varied with CO₂ in phase but with O₂ out-of phase; (ii) CO₂ flux was dominated initially by anaerobic discharge and subsequently by aerobic products; (iii) linear relationships between aerobic reheating and both O₂ consumption (0.994 ≥ R² ≥ 0.815, P < 0.01) and CO₂ flux (0.981 ≥ R² ≥ 0.464, P < 0.01); and (iv) variable magnitude of daily aerobic production of CO₂ per kg DM from 2.3 to 133.4 mmol kg d⁻¹. These results demonstrate that the novel multi-sensor technique has powerful capacity to provide insight into AMR of silage and thus provide more detailed information to guide silage management than previous measurements of aerobic stability.

有氧微生物呼吸（AMR）的生化反应表明，青贮饲料温度（T _si）升高，氧气（O ₂）消耗和二氧化碳（CO ₂）排放可以作为饲喂期间青贮饲料劣化的指标，但仅温度已被广泛用于评估有氧稳定性。在这里，我们通过开发集成了多个传感器的新型实验细胞，扩展了对有氧稳定性的研究，以结合青贮饲料的AMR。用小黑麦作物青贮的青贮饲料样品在十二个气密桶（60 L）中制成，包装成190或250 kg m ^-3干物质（DM）的堆积密度。T _si和O ₂测量值位于工作面后15厘米和30厘米处。将CO ₂测量为穿过工作面的通量。有氧暴露的实验期为7天。我们提供了以下第一份报告：（i）这些参数的不同的有氧响应，表明T _si随同相CO ₂而异相随O ₂变化；（ii）CO ₂通量主要由厌氧排放控制，随后由需氧产物控制；（ⅲ）线性有氧再加热和均为O之间的关系的₂消耗（0.994≥ - [R ²  ≥0.815，P <0.01）和CO ₂通量（0.981≥ - [R ² ≥0.464，P <0.01）; （iv）每公斤DM的每日有氧生产CO _2的变化幅度从2.3到133.4 mmol kg d ^-1。这些结果表明，与以前的有氧稳定性测量相比，新颖的多传感器技术具有强大的能力，可洞悉青贮饲料的AMR，因此可提供更详细的信息来指导青贮饲料的管理。