Non-tumorigenic Rhizobium (syn. Agrobacterium) vitis strain ARK-1 (ARK-1) has been shown to reduce crown gall in grapevine (Vitis vinifera) seedlings when co-inoculated with Japanese tumorigenic isolates of R. vitis. The objectives of this study were to test ARK-1's efficacy as a biological control agent against R. vitis isolated from grapevines in Virginia, and to examine genetic diversity of R. vitis found in Virginia, USA and Japan. ARK-1 was co-inoculated into wine grape trunks (V. vinifera cv. ‘Cabernet Sauvignon’) with a tumorigenic R. vitis isolate at a 1:1 cell ratio (~10⁸ cell/ml). A total of four R. vitis isolates were tested individually. Compared to treatments of a tumorigenic isolate alone, ARK-1 co-inoculation significantly decreased (P ≤ 0.05) the mean probability of gall formation in all cases, and the mean gall diameter in all, but one case (isolate ACME15). The average reduction was 90% and 92% in the mean probability of gall formation and mean gall diameter, respectively. We conducted exploratory data visualization with distance-based clustering and correspondence analysis using data from ISSR- and rep-PCR, which showed separation of Virginia and Japanese isolates and placement of ARK-1 near to Virginia isolates. These results suggest that ARK-1 is a good candidate for biocontrol of grapevine crown gall in the mid-Atlantic region of the USA.

与日本致病性葡萄球菌分离株共同接种时，非致瘤性根瘤菌（土壤杆菌属）葡萄树菌株ARK-1（ARK-1）已显示出能够减少葡萄树（Vitis vinifera）幼苗中的冠gall病。这项研究的目的是测试ARK-1的效果作为对生物控制剂R.葡萄在葡萄弗吉尼亚州的分离，并研究遗传多样性R.葡萄在弗吉尼亚州，美国和日本发现。将ARK-1与致瘤性葡萄球菌分离株以1：1的细胞比例（〜10 ⁸）一起接种到酿酒葡萄树干（V. vinifera cv。'Cabernet Sauvignon'）中。 细胞/毫升）。共有4个R.葡萄分离物单独测试。相比于单独的致瘤分离物的治疗，ARK-1共接种显著降低（P  ≤0.05）的在所有情况下瘿形成的平均概率，并在所有的平均瘿直径，但一种情况下（分离物ACME15）。胆结形成的平均概率和胆平均直径的平均减少分别为90％和92％。我们使用基于距离的聚类进行了探索性数据可视化，并使用来自ISSR和rep-PCR的数据进行了对应分析，结果表明弗吉尼亚和日本分离株的分离以及ARK-1在弗吉尼亚分离株附近的放置。这些结果表明，ARK-1是美国中大西洋地区葡萄冠gall生物防治的良好候选者。