当前位置: X-MOL 学术Protein Expres. Purif. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Characterization of recombinant E. coli expressing a novel fucosidase from Bacillus cereus 2–8 belonging to GH95 family
Protein Expression and Purification ( IF 1.6 ) Pub Date : 2021-05-12 , DOI: 10.1016/j.pep.2021.105897
Qian Li 1 , Chaofeng Jiang 2 , Haidong Tan 3 , Xiaoming Zhao 3 , Kuikui Li 3 , Heng Yin 3
Affiliation  

Fucoidan oligosaccharides possesses diverse physicochemical and biological activities. Specific glycoside hydrolases are valuable tools for degrading polysaccharides to produce oligosaccharides. In this study, BcFucA, a novel fucosidase belonging to GH95 family from Bacillus cereus 2–8, was cloned into pET21a vector, expressed in E. coli BL21 (DE3) and characterized. The protein consists of 1136 amino acid residues encoded by 3411 bases and has a molecular weight of 125.35 kDa. The optimal temperature and pH of this enzyme are 50 °C and pH 4.0. In addition, this study showed that the unknown function domain (encoding Lys261-Thr681) defined as a linker is quite important for its activity. The obtained novel enzyme BcFucA will contribute to the effective degradation of fucoidan and future industrial applications.



中文翻译:

重组大肠杆菌的表征从属于 GH95 家族的蜡状芽孢杆菌 2-8 中表达一种新型岩藻糖苷酶

褐藻糖胶寡糖具有多种物理化学和生物活性。特定的糖苷水解酶是用于降解多糖以产生寡糖的有价值的工具。在这项研究中,Bc FucA 是一种来自蜡状芽孢杆菌2-8的 GH95 家族的新型岩藻糖苷酶,被克隆到 pET21a 载体中,在大肠杆菌BL21 (DE3) 中表达并进行了表征。该蛋白质由3411个碱基编码的1136个氨基酸残基组成,分子量为125.35 kDa。这种酶的最佳温度和 pH 值为 50 °C 和 pH 4.0。此外,这项研究表明,定义为接头的未知功能域(编码 Lys261-Thr681)对其活性非常重要。获得的新型酶BcFucA 将有助于褐藻糖胶的有效降解和未来的工业应用。

更新日期:2021-05-19
down
wechat
bug