Purpose

The relationship between Hashimoto's thyroiditis and vitamin D concentration was already presented in many studies. The aim of this study was to analyze the differences in the concentration of vitamin D metabolites between healthy women and women with Hashimoto's thyroiditis (HT).

Methods

The quantitative analysis of five vitamin D metabolites was carried out using liquid chromatography coupled with tandem mass spectrometry. The analyzed materials were serum and dried blood spots (DBS). The results obtained for the two materials were also compared.

Results

No statistically significant differences were found in the mean concentration of the 25(OH)D₃, 25(OH)D₂, 24,25(OH)₂D₃, 1,25(OH)₂D₃ metabolites between the test and the control groups. However, a strong correlation was found between the 25(OH)D₃ and 24,25(OH)₂D₃ metabolites.

Conclusion

The study showed that healthy women and women with Hashimoto's disease had similar concentration of vitamin D metabolites. Research also proved that DBS is a good alternative to serum. The differences in 25(OH)D concentration were not statistically significant (17.0 and 15.5 ng mL⁻¹ for serum and DBS, respectively). DBS can be successfully used in research on a large group of people, since the process of material collection, as well as sample preparation, is fast and simple. It is also easy to transport and store, and requires small volume of blood.

中文翻译：

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干血斑和血清样品在健康女性和桥本甲状腺炎人群中维生素D代谢物测定中的应用

目的

许多研究已经提出了桥本甲状腺炎与维生素D浓度之间的关系。这项研究的目的是分析健康女性和桥本甲状腺炎（HT）女性之间维生素D代谢物浓度的差异。

方法

使用液相色谱和串联质谱法对5种维生素D代谢产物进行了定量分析。被分析的材料是血清和干血斑（DBS）。还比较了两种材料获得的结果。

结果

没有统计学上在25（OH）d的平均浓度被发现显著差异₃，25（OH）d ₂，24,25（OH）₂ d ₃，1,25（OH）₂ d _3组的试验之间的代谢物和对照组。但是，在25（OH）D ₃和24,25（OH）₂ D ₃代谢产物之间发现了很强的相关性。

结论

研究表明，健康的女性和桥本氏病的女性维生素D代谢产物的浓度相似。研究还证明，DBS是血清的良好替代品。25（OH）D浓度的差异无统计学意义（血清和DBS分别为17.0和15.5 ng mL ^-1）。DBS可以成功地用于一大群人的研究中，因为材料收集和样品制备过程既快速又简单。它也易于运输和存储，并且需要少量的血液。