当前位置: X-MOL 学术Front. Life Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Editing sterol side chain reductase 2 gene (StSSR2) via CRISPR/Cas9 reduces the total steroidal glycoalkaloids in potato
Frontiers in Life Science ( IF 1.333 ) Pub Date : 2021-05-12 , DOI: 10.1080/26895293.2021.1925358
Zhenzhen Zheng 1, 2, 3, 4 , Guangji Ye 2, 3, 4, 5 , Yun Zhou 2, 3, 4, 5 , Xiuqin Pu 2, 3, 4, 5 , Wang Su 2, 3, 4, 5 , Jian Wang 2, 3, 4, 5
Affiliation  

Steroidal glycoalkaloids (SGAs) existing in most potato tissues are toxic to humans when the fresh weight is over 200 mg kg−1, and high SGAs content would also damage the quality of potato tubers. Sterol side chain reductase 2 (StSSR2) is a key enzyme in the biosynthetic of SGAs in potatoes. To reduce the concentration of SGAs in tetraploid cultivated potato (Solanum tuberosum L.), the gene StSSR2 was edited by the CRISPR/Cas9 system. A total number of 64 mutant lines were generated, and the mutation efficiency was 46%. The percentage of insertion was 6.25%, and the percentage of deletion was 93.75%. Compared to the wild-type (WT), the content of SGAs reduced significantly in the mutated potatoes. The lowest SGAs concentration was 54% of the WT level, which was recorded in the peels of line #360. In the tuber fleshes, the lowest SGAs were 66% of WT level, which was found in line #378. In the leaves, the lowest SGAs concentration was 44% of the WT level as found in lines #204 and #378. In a word, our results showed that the StSSR2 gene of tetraploid cultivated potato could be successfully edited by the CRISPR/Cas9 system to reduce toxic SGAs.



中文翻译:

通过CRISPR / Cas9编辑固醇侧链还原酶2基因(StSSR2)可减少马铃薯中的总甾体类生物碱

当鲜重超过200 mg kg -1时,大多数马铃薯组织中存在的甾体类生物碱(SGA)对人体有毒,而高SGA含量也会损害马铃薯块茎的质量。甾醇侧链还原酶2(StSSR2)是马铃薯SGA生物合成中的关键酶。为了降低四倍体栽培马铃薯(Solanum tuberosum L.)中SGA的浓度,基因StSSR2由CRISPR / Cas9系统编辑。总共产生64个突变体系,并且突变效率为46%。插入百分比为6.25%,删除百分比为93.75%。与野生型(WT)相比,突变马铃薯中SGA的含量显着降低。最低的SGA浓度为WT水平的54%,记录在#360线的果皮中。在块茎肉中,最低的SGA为野生型水平的66%,在第378行发现。在叶片中,最低的SGA浓度为#204和#378品系中WT水平的44%。总之,我们的结果表明,可以通过CRISPR / Cas9系统成功编辑四倍体栽培马铃薯的StSSR2基因,以减少有毒的SGA。

更新日期:2021-05-12
down
wechat
bug