This study aimed to examine the expression and clinical significance of chemokine-like factor-like MARVEL transmembrane domain-containing family member 8 (CMTM8) in gastric cancer (GC). The mRNA and protein expression of CMTM8 were detected by bioinformatics analysis and immunohistochemistry (IHC), respectively. Bioinformatics analysis found that there was a high mRNA expression of CMTM8 in GC tissues, but failed to identify a significant relationship with the clinicopathological features or prognosis of GC patients. However, IHC results showed that the positive expression of CMTM8 protein in GC tissues was significantly lower than that of adjacent nontumor tissues and correlated with differentiation, tumor node metastasis stage, and distal metastasis of GC patients (p < 0.05). Moreover, the survival time of GC patients with negative CMTM8 protein expression group was shorter than that of positive CMTM8 protein expression group by Kaplan–Meier survival analysis (p < 0.05). Cox proportional hazards model (COX) regression analysis indicated that CMTM8 protein was an independent protective factor for the overall survival of GC patients. Further Gene Set Enrichment Analysis suggested that CMTM8 may be involved in regulating the calcium signaling pathway, cell adhesion molecules, and cytokine–cytokine receptor interaction in GC. Our study shows that CMTM8 protein is downregulated in GC tissues, and CMTM8 protein expression is related to GC metastasis and the prognosis of GC patients.

中文翻译：

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下调 CMTM8 与胃癌患者的不良预后相关

本研究旨在研究趋化因子样因子样 MARVEL 跨膜结构域家族成员 8 (CMTM8) 在胃癌 (GC) 中的表达和临床意义。分别通过生物信息学分析和免疫组织化学（IHC）检测CMTM8的mRNA和蛋白表达。生物信息学分析发现，CMTM8在GC组织中有高mRNA表达，但未能确定与GC患者的临床病理特征或预后有显着关系。但IHC结果显示CMTM8蛋白在GC组织中的阳性表达显着低于邻近非肿瘤组织，并与GC患者的分化、肿瘤淋巴结转移分期、远端转移相关（p < 0.05)。此外，通过Kaplan-Meier生存分析，CMTM8蛋白表达阴性组GC患者的生存时间短于CMTM8蛋白表达阳性组（p  < 0.05）。Cox比例风险模型（COX）回归分析表明，CMTM8蛋白是GC患者总生存期的独立保护因素。进一步的基因集富集分析表明，CMTM8 可能参与调节 GC 中钙信号通路、细胞粘附分子和细胞因子-细胞因子受体相互作用。我们的研究表明CMTM8蛋白在GC组织中下调，CMTM8蛋白表达与GC转移和GC患者的预后有关。