BCG is widely used for cancer treatment, where macrophages play an important role. However, the mechanism of BCG affecting macrophages remains poorly understood. In this study, we used BCG to stimulate myeloid-derived macrophages lacking HIF-1α, the levels of TNF-α, IL-1β, CD86 of macrophages and their effects on the growth of tumor cells MCA207 and B16-F10 were detected. We found that the absence of HIF-1α prevents BCG-stimulated macrophages from polarizing towards the M (BCG) and attenuating its killing effect on tumor cells. In addition, we demonstrated that the tumors of mice lacking HIF-1α in macrophages were significantly increased by the experiment of mice transplantation. Our study provides relevant evidence for exploring the mechanism of the BCG vaccine in the prevention and treatment of related diseases.

BCG 广泛用于癌症治疗，其中巨噬细胞起着重要作用。然而，卡介苗影响巨噬细胞的机制仍然知之甚少。本研究使用卡介苗刺激缺乏 HIF-1α 的髓源性巨噬细胞，检测巨噬细胞 TNF-α、IL-1β、CD86 的水平及其对肿瘤细胞 MCA207 和 B16-F10 生长的影响。我们发现 HIF-1α 的缺失可防止 BCG 刺激的巨噬细胞向 M (BCG) 极化并减弱其对肿瘤细胞的杀伤作用。此外，我们还通过小鼠移植实验证明了巨噬细胞中缺乏HIF-1α的小鼠的肿瘤显着增加。本研究为探索卡介苗预防和治疗相关疾病的机制提供了相关证据。