This study involved the use of aqueous extract from grapes as reducing substance for the green synthesis of NiO nanoparticles (NiONPs). The morphology, crystalline structure and elementary composition were investigated through various characterisation methods, such as X-ray diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and UV–Visible spectroscopy, to demonstrate the synthesis of pure NiONPs. Strong anti-bacterial inhibitory activities of NiONPs have been examined by pathogenic bacterial strains studied against Gram-negative bacterial strain Klebsiella pneumoniae and Gram-positive bacterial strain Staphylococcus aureus. Potential cytotoxicity against human breast cancer (MCF-7) and human brain cancer (AMGM5) cell lines was shown by NiONPs. NiONPs have shown excellent biological applications. Moreover, NiO nanoparticles exhbibit toxicity to be safely used for different biomedical applications.

这项研究涉及使用葡萄的水提取物作为还原物质，用于绿色合成 NiO 纳米粒子 (NiONPs)。通过各种表征方法（如 X 射线衍射、扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱和紫外-可见光谱）研究了形貌、晶体结构和元素组成，以证明纯 NiONP 的合成。NiONPs 的强抗菌抑制活性已通过针对革兰氏阴性菌肺炎克雷伯菌和革兰氏阳性菌金黄色葡萄球菌研究的病原菌菌株进行检验. NiONPs 显示出对人乳腺癌 (MCF-7) 和人脑癌 (AMGM5) 细胞系的潜在细胞毒性。NiONPs 已显示出优异的生物学应用。此外，NiO 纳米颗粒表现出毒性，可安全用于不同的生物医学应用。