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Transcriptome analysis of resistance mechanism to potato wart disease
Open Life Sciences ( IF 2.2 ) Pub Date : 2021-01-01 , DOI: 10.1515/biol-2021-0045
Peihua Li 1 , Ruihong Fan 2 , Zhengsong Peng 1 , Yuan Qing 1 , Zhirong Fang 1
Affiliation  

To understand the molecular mechanism of the resistance to potato wart disease, we used the potato cultivar Qingshu 9 as the experimental material and prepared potato samples with different levels of disease through inoculation. The RNAs of the samples were extracted, and transcriptome analysis was performed on the samples not infected by the disease (control group) and also on the samples with different levels of disease, with the aid of high-throughput sequencing. Next, the differentially expressed genes (DEGs) associated with the resistance to potato wart disease were identified based on the analysis results. Using bioinformatic tools, the DEGs were functionally annotated, classified, and enriched in related metabolic pathways. The main results are as follows: Compared with the control group, 4 DEGs were identified in the samples with light disease, 52 were found in the samples with medium disease, and 214 were discovered in the samples with heavy disease. Potato mainly resists the wart disease by suppressing its gene expression, and the degree of suppression depends on the level of the disease; the disease resistance might be dominated by cellular nucleic acid-binding protein, AP2-like transcription factor, and E3 ubiquitin-protein ligase. This research provides new insights into the molecular mechanism of potato resistance against wart disease.

中文翻译:

马铃薯疣病抗性机制的转录组分析

为了了解抗马铃薯疣病的分子机制,我们以马铃薯品种清薯9号为实验材料,并通过接种制备了不同病害水平的马铃薯样品。提取样品的RNA,并借助高通量测序对未受疾病感染的样品(对照组)以及具有不同疾病水平的样品进行转录组分析。接下来,基于分析结果,鉴定了与对马铃薯疣病的抗性相关的差异表达基因(DEG)。使用生物信息学工具,对DEG进行功能注释,分类并丰富相关的代谢途径。主要结果如下:与对照组相比,在轻病样本中鉴定出4种DEG,在中病样本中发现52种,在重病样本中发现214种。马铃薯主要通过抑制其基因表达来抵抗疣病,其抑制程度取决于病害的程度。抗病性可能由细胞核酸结合蛋白,AP2样转录因子和E3泛素蛋白连接酶控制。这项研究为马铃薯抗疣病的分子机制提供了新的见解。抗病性可能由细胞核酸结合蛋白,AP2样转录因子和E3泛素蛋白连接酶控制。这项研究为马铃薯抗疣病的分子机制提供了新的见解。抗病性可能由细胞核酸结合蛋白,AP2样转录因子和E3泛素蛋白连接酶控制。这项研究为马铃薯抗疣病的分子机制提供了新的见解。
更新日期:2021-01-01
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