An exceptionally simple and versatile advance in super-resolution microscopy has been created by adding a new birefringent FINCH holographic lens system including an inexpensive uncooled CMOS camera to a standard microscope. Resolution, after only a single image capture, is equivalent to or better than other more complex popular methods such as SIM, Airyscan and a number of image scanning microscopy methods that boost resolution about two-fold. This new FINCH implementation uniquely works for any objective power and NA and is solid state, fast, and calibration-free. In addition to being as easy to operate and maintain as a standard fluorescence microscope, it can uniquely create super-resolved images with any type or wavelength of light including fluorescence, bioluminescence or reflected light because its principle depends only on emitted light from objects and requires no prior training or knowledge about the sample being imaged. This microscope technique increases the utility and availability of super-resolution microscopy for any user in any research lab.

中文翻译：

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单次拍摄全息超分辨率显微镜

通过在标准显微镜上添加新的双折射FINCH全息透镜系统（包括便宜的未冷却CMOS相机），在超分辨率显微镜领域取得了异常简单和通用的进步。仅捕获一次图像后，分辨率就等于或优于其他更复杂的常用方法，例如SIM，Airescan和许多将分辨率提高两倍的图像扫描显微技术。这种新的FINCH实现特别适用于任何目标功率和NA，并且是固态，快速且无需校准的。除了像标准的荧光显微镜一样易于操作和维护之外，它还可以独特地创建具有任何类型或波长的光（包括荧光，生物发光或反射光，因为其原理仅取决于物体发出的光，并且不需要事先培训或对要成像的样品的知识。这种显微镜技术为任何研究实验室中的任何用户增加了超分辨率显微镜的实用性和可用性。