Abstract

Purpose

Transcriptomic-based approaches are being developed to meet the needs for large-scale radiation dose and injury assessment and provide population triage following a radiological or nuclear event. This review provides background and definition of the need for new biodosimetry approaches, and summarizes the major advances in this field. It discusses some of the major model systems used in gene signature development, and highlights some of the remaining challenges, including individual variation in gene expression, potential confounding factors, and accounting for the complexity of realistic exposure scenarios.

Conclusions

Transcriptomic approaches show great promise for both dose reconstruction and for prediction of individual radiological injury. However, further work will be needed to ensure that gene expression signatures will be robust and appropriate for their intended use in radiological or nuclear emergencies.

Sally Amundson is an Associate Professor in the Center for Radiological Research at Columbia University Irving Medical Center in New York. She was graduate student #28 in Jack Little’s laboratory.

中文翻译：

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辐射生物剂量学的转录组学：进展与挑战

摘要

目的

正在开发基于转录组学的方法，以满足大规模辐射剂量和伤害评估的需要，并在放射学或核事件后提供人群分类。本综述提供了需要新的生物剂量学方法的背景和定义，并总结了该领域的主要进展。它讨论了基因签名开发中使用的一些主要模型系统，并强调了一些剩余的挑战，包括基因表达的个体差异、潜在的混杂因素以及对现实暴露场景的复杂性的解释。

结论

转录组学方法在剂量重建和个体放射损伤预测方面显示出巨大前景。然而，还需要进一步的工作来确保基因表达特征是稳健的并且适合它们在放射学或核紧急情况下的预期用途。

Sally Amundson 是纽约哥伦比亚大学欧文医学中心放射学研究中心的副教授。她是 Jack Little 实验室的第 28 号研究生。