The present study was designed to the synthesis of pH- responsive silver nanoparticles (AgNPs) by green route approach using Labeo rohita (L. rohita) fish scale aqueous extract. The synthesized AgNPs were optimized by various pH (5–9) ranges and characterized by Ultraviolet–Visible spectroscopy (UV–Vis), X-ray diffraction crystallography (XRD), Fourier transform infrared spectroscopy (FT-IR), Transmission electron microscopy (TEM), Zeta potential, and dynamic light scattering (DLS) analysis. Surface Plasmon Resonance (SPR) bands observed at 400–405 nm range in UV–Visible spectrum and this SPR band was preliminary confirmation of AgNPs synthesis. FT-IR spectral results show that different functional groups acted as definitive reduction and capping agents for the formation of AgNPs. Microscopy analysis confirm that AgNPs are spherical in shape with average particle size of 42.9 to 80.1 nm in range and confirmed that the 65.19% of Ag ions present in AgNPs by EDAX analysis. In addition, the antimicrobial activity of AgNPs revealed potential activity against different pathogens. Among those pathogens, Staphylococcous aureus showed higher zone of inhibition (8.2 ± 1.4 mm) at 100 μg/mL. In addition, S. aureus inhibition was proved by SEM morphological analysis, which indicates cell wall damaged. Overall, this present investigation confirms that green route AgNPs are potential source for biomedical applications.

本研究旨在使用Labeo rohita（L. rohita）的绿色路线方法合成pH响应性银纳米颗粒（AgNPs）。）鱼鳞水提物。合成的AgNPs在各种pH值（5–9）范围内均经过优化，并具有紫外-可见光谱（UV-Vis），X射线衍射晶体学（XRD），傅里叶变换红外光谱（FT-IR），透射电子显微镜（ TEM），Zeta电位和动态光散射（DLS）分析。在紫外可见光谱中在400–405 nm范围内观察到的表面等离子体共振（SPR）谱带，该SPR谱带初步证实了AgNPs的合成。FT-IR光谱结果表明，不同的官能团可作为确定的还原剂和封端剂来形成AgNPs。显微镜分析证实AgNP为球形，平均粒径范围为42.9至80.1nm，并通过EDAX分析证实AgNP中存在65.19％的Ag离子。此外，AgNPs的抗菌活性揭示了其对不同病原体的潜在活性。在这些病原体中，葡萄球菌的金黄色葡萄球菌在100μg/ mL处显示出较高的抑制区（8.2±1.4 mm）。另外，通过SEM形态分析证明了金黄色葡萄球菌的抑制，表明细胞壁受损。总的来说，本研究证实绿色途径的AgNPs是生物医学应用的潜在来源。