Microorganisms play an essential role in the growth and development of numerous insect species. In this study, the total DNA from the midgut of adults of Dactylispa xanthospila were isolated and bacterial 16S rRNA sequenced using the high-throughput Illumina MiSeq platform. Then, the composition and diversity of the midgut bacterial community were analysed with QIIME2. The results showed the midgut bacteria of D. xanthospila belong to 30 phyla, 64 classes, 135 orders, 207 families and 369 genera. At the phylum level, Proteobacteria, Bacteroidetes and Firmicutes were the dominant bacteria, accounting for 91.95%, 3.44% and 2.53%, respectively. The top five families are Enterobacteriaceae (69.51%), Caulobacteraceae (5.24%), Rhizobiaceae (4.61%), Sphingomonadaceae (4.23%) and Comamonadaceae (2.67%). The bacterial community's primary functions are carbohydrate metabolism, amino acid metabolism and cofactor and vitamin metabolism, which are important for the nutritional requirements of plant-feeding insects.

中文翻译：

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采叶甲虫Dactylispa xanthospila（Gestro）（鞘翅目：金眼科：Cassidinae）的中肠细菌多样性

微生物在众多昆虫物种的生长和发育中起着至关重要的作用。在这项研究中，分离出了来自Dactylispa xanthospila的成虫中肠的总DNA，并使用高通量Illumina MiSeq平台对细菌16S rRNA进行了测序。然后，用QIIME2分析中肠细菌群落的组成和多样性。结果表明：黄果石mid中肠细菌属于30门，64类，135目，207科，369属。在门类中，变形杆菌，拟杆菌和硬菌是最主要的细菌，分别占91.95％，3.44％和2.53％。排在前五位的是肠杆菌科（69.51％），杆状杆菌科（5.24％），根瘤菌科（4.61％），Sphingomonadaceae（4.23％）和Comamonadaceae（2.67％）。细菌群落