The intensive aquaculture strategy and recirculating aquaculture system often lead to the production of off-flavor compounds such as 2-methyl-isoborneol (2-MIB) and Geosmin (GSM). The regular purge and trap extraction followed by analysis with gas chromatography-mass spectrometry (GC-MS) usually involve a complicated assembly of facilities, more working space, long sample preparation time, and headspace solid-phase microextraction (SPME). In this work, a method with easier sample preparation, fewer and simplified facilities, and without SPME on GC-MS analysis is developed for the determination of 2-MIB and GSM in fish samples. Unlike previous methods, solvent extract from samples, QuEChERS-based cleanup, and solid-phase extraction for concentration are applied. The LOD (S/N > 3) and LOQ (S/N > 10) of this method were validated at 0.6 μg/kg and 1.0 μg/kg for both 2-MIB and GSM, which are under the sensory limit (1 μg/kg). Application of this method for incurred fish samples demonstrated acceptable analytical performance. This method is suitable for large-scale determination of 2-MIB and GSM in fish samples, owing to the use of simple facility and easy-to-operate procedure, rapid sample preparation, and shorter time for GC-MS analysis without SPME.

中文翻译：

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一种有效的鱼类中2-甲基异冰片和土臭素的样品制备方法及其气相色谱-质谱分析

集约化水产养殖策略和循环水产养殖系统经常导致产生异味化合物，例如2-甲基异冰片醇（2-MIB）和土臭味素（GSM）。常规的吹扫捕集萃取，然后进行气相色谱-质谱分析（GC-MS），通常需要复杂的设备组装，更多的工作空间，较长的样品制备时间以及顶空固相微萃取（SPME）。在这项工作中，开发了一种方法，该方法可简化样品制备，减少设备并简化操作，并且无需在GC-MS分析上使用SPME即可测定鱼样品中的2-MIB和GSM。与以前的方法不同，采用了从样品中提取溶剂，基于QuEChERS的净化方法和固相提取法进行浓缩的方法。该方法的LOD（S / N> 3）和LOQ（S / N> 10）验证为0.6 μ克/公斤和1.0  μ克/公斤两个2-MIB和GSM，其是下感官极限（1  μ克/千克）。该方法在已发生鱼类样品中的应用证明了可接受的分析性能。该方法适用于鱼类样品中的2-MIB和GSM的大规模测定，这是因为它使用了简单的仪器，易于操作的程序，快速的样品前处理和更短的GC-MS分析时间（无需SPME）。