Thirteen jute genotypes were evaluated for resistance against Macrophomina phaseolina (Mp) in the sick plot, and six of them were identified for stable differential disease reactions. We tested whether other screening methods could produce similar results in these host genotypes. An incubation temperature of 35 °C was optimum for Mp sclerotia germination (90.8%), and it was chosen for experiments involving sclerotia as inoculum. Infestation with 250 or more Mp sclerotia/g seed induced morbidity in ~ 40% seedlings in vitro. Inoculation at the root and leaf could not differentiate between susceptible (JRO 524) and resistant (JRO 204) cultivars. Infesting both growth media and seed with Mp sclerotia induced early (15 days post-inoculation, DPI) expression of disease symptoms. However, the host reactions were in great variation with those from the field screening. Mp inoculum consisting of growing mycelial tips was more aggressive producing 6.4 cm long stem lesion at 4 DPI as compared to 3.9 cm by 7-day-old inoculum. Inoculating at the middle of the main stem produced the largest lesion (5.7 cm long) compared to the basal (3.3 cm) and the tip (1.3 cm) inoculations 6 DPI. Plants at ≥ 70 days after sowing (DAS) produced larger stem lesions than younger plants. This technique grouped test genotypes similar to sick plot experiments, with minor variations. Stem inoculation method may be adopted for large-scale screening and also confirming the resistance reaction of jute towards Mp infection.

评估了13种黄麻基因型对菜豆巨噬菌的抵抗力（Mp），并确定其中有6个用于稳定的差异性疾病反应。我们测试了其他筛选方法是否可以在这些宿主基因型中产生相似的结果。35 M的培养温度最适合Mp菌核萌发（90.8％），并且选择将菌核作为接种物进行实验。250或更多Mp菌核/克种子的侵染可在约40％的幼苗中诱发发病。在根部和叶部的接种无法区分易感（JRO 524）和抗性（JRO 204）品种。用Mp菌核侵染生长培养基和种子会诱导疾病症状的早期表达（接种后15天，DPI）。但是，宿主反应与现场筛选的反应差异很大。Mp接种物由不断增长的菌丝体尖端组成，在4 DPI时产生6.4 cm长的茎部病变，而7天龄的接种物为3.9 cm。在主茎中部接种产生最大的病灶（5.7厘米长），而在基部接种6 DPI则产生最大的病灶（3.3厘米）和尖端（1.3厘米）。播种后≥70天（DAS）的植物比年轻植物产生更大的茎损伤。该技术将测试基因型与病态实验相似，但变化很小。可以采用茎接种方法进行大规模筛选，也可以确认黄麻对Mp感染的耐药性反应。3厘米）接种6 DPI。播种后≥70天（DAS）的植物比年轻植物产生更大的茎损伤。该技术将测试基因型与病态实验相似，但变化很小。可以采用茎接种方法进行大规模筛选，也可以确认黄麻对Mp感染的耐药性反应。3厘米）接种6 DPI。播种后≥70天（DAS）的植物比年轻植物产生更大的茎损伤。该技术将测试基因型与病态实验相似，但变化很小。可以采用茎接种方法进行大规模筛选，也可以确认黄麻对Mp感染的耐药性反应。