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55 Sow Milk Lipidome Study Reveals Changes in Fatty Acyl Residues in Triglycerides and Phosphatidylglycerol, but Not in Plasma Membrane Phospholipids Across Lactation
Journal of Animal Science ( IF 3.3 ) Pub Date : 2021-05-08 , DOI: 10.1093/jas/skab054.261 Sarah Luecke 1 , Aridany Suarez-Trujillo 1 , Lea Logan 1 , Kara R Stewart 1 , Christina Ferreira 2 , Theresa Casey 1
Journal of Animal Science ( IF 3.3 ) Pub Date : 2021-05-08 , DOI: 10.1093/jas/skab054.261 Sarah Luecke 1 , Aridany Suarez-Trujillo 1 , Lea Logan 1 , Kara R Stewart 1 , Christina Ferreira 2 , Theresa Casey 1
Affiliation
Sow milk fat content is crucial to neonatal survival, as it is utilized for thermogenesis and nutrition. However, fat is the most variable component of milk in concentration and lipid species. Characterizing lipid changes across the course of a sow’s lactation may help identify molecules or systems to target to help enhance milk fat quality and quantity for neonatal survival and growth. Percent fat variation is greatest in colostrum, the first milk. Little is known regarding colostrum synthesis, other than it accumulates in the gland beginning in mid-late pregnancy, which is prior to the initiation of fatty acid synthesis in lactocytes. The objective of this study was to characterize changes in lipid composition of milk across the course of lactation and determine if there was a relationship between fat percent and lipid species in colostrum and mature milk. Milk was collected from 9 multiparous sows on days 0, 3, 7, and 14 relative to birth. Percent fat was determined by creamatocrit, and found to be different (p< 0.05) between day 0 (12.36 ± 5.90%) and day 3 (16.22 ± 3.65%) but not between day 7 (13.13 ± 2.19%) and 14 (12.13 ± 2.45%). Fat was extracted from milk using the Bligh-Dyer method and profiled using multiple reaction monitoring. Amounts of lipid species were calculated relative to standards and data analysis was performed using Metaboanalyst 4.0. Principle component analysis revealed lactation day had a significant effect on distribution of fats. Triacylglycerides (TAG), phosphatidylglycerol (PG), and plasma membrane lipids were modified from colostrum to mature milk, with a significant increase in PGs and TAGs across the course of lactation. Correlation analysis of percent fat with lipid concentration indicated strong relationships (P < 0.05; |r| >0.80) with eight lipids. No differences are found in the abundance of plasma membrane phospholipids, sphingomyelin, or cholesterol esters across lactation days.
中文翻译:
55 母猪牛奶脂质组研究揭示了在整个哺乳期的甘油三酯和磷脂酰甘油中脂肪酰基残基的变化,但在血浆膜磷脂中没有变化
母猪乳脂含量对新生儿存活至关重要,因为它用于产热和营养。然而,脂肪是牛奶中浓度和脂质种类变化最大的成分。表征母猪泌乳过程中的脂质变化可能有助于确定目标分子或系统,以帮助提高乳脂的质量和数量,以促进新生儿的存活和生长。初乳中的脂肪百分比变化最大,初乳。关于初乳合成知之甚少,除了它在妊娠中后期开始在腺体中积累,这是在乳细胞中脂肪酸合成开始之前。本研究的目的是描述整个泌乳过程中牛奶脂质成分的变化,并确定初乳和成熟乳中的脂肪百分比与脂质种类之间是否存在关系。在相对于出生的第 0、3、7 和 14 天从 9 头经产母猪收集牛奶。脂肪百分比由乳脂比容测定,发现在第 0 天 (12.36 ± 5.90%) 和第 3 天 (16.22 ± 3.65%) 之间存在差异 (p<0.05),但在第 7 天 (13.13 ± 2.19%) 和第 14 天 (12.13) 之间没有差异± 2.45%)。使用 Bligh-Dyer 方法从牛奶中提取脂肪,并使用多反应监测进行分析。相对于标准计算脂质种类的量,并使用 Metaboanalyst 4.0 进行数据分析。主成分分析显示,哺乳日对脂肪分布有显着影响。三酰基甘油酯 (TAG)、磷脂酰甘油 (PG) 和质膜脂质从初乳变为成熟乳,在整个哺乳过程中 PG 和 TAG 显着增加。脂肪百分比与脂质浓度的相关性分析表明与八种脂质有很强的相关性(P < 0.05;|r| > 0.80)。在整个哺乳期,质膜磷脂、鞘磷脂或胆固醇酯的丰度没有发现差异。
更新日期:2021-05-08
中文翻译:
55 母猪牛奶脂质组研究揭示了在整个哺乳期的甘油三酯和磷脂酰甘油中脂肪酰基残基的变化,但在血浆膜磷脂中没有变化
母猪乳脂含量对新生儿存活至关重要,因为它用于产热和营养。然而,脂肪是牛奶中浓度和脂质种类变化最大的成分。表征母猪泌乳过程中的脂质变化可能有助于确定目标分子或系统,以帮助提高乳脂的质量和数量,以促进新生儿的存活和生长。初乳中的脂肪百分比变化最大,初乳。关于初乳合成知之甚少,除了它在妊娠中后期开始在腺体中积累,这是在乳细胞中脂肪酸合成开始之前。本研究的目的是描述整个泌乳过程中牛奶脂质成分的变化,并确定初乳和成熟乳中的脂肪百分比与脂质种类之间是否存在关系。在相对于出生的第 0、3、7 和 14 天从 9 头经产母猪收集牛奶。脂肪百分比由乳脂比容测定,发现在第 0 天 (12.36 ± 5.90%) 和第 3 天 (16.22 ± 3.65%) 之间存在差异 (p<0.05),但在第 7 天 (13.13 ± 2.19%) 和第 14 天 (12.13) 之间没有差异± 2.45%)。使用 Bligh-Dyer 方法从牛奶中提取脂肪,并使用多反应监测进行分析。相对于标准计算脂质种类的量,并使用 Metaboanalyst 4.0 进行数据分析。主成分分析显示,哺乳日对脂肪分布有显着影响。三酰基甘油酯 (TAG)、磷脂酰甘油 (PG) 和质膜脂质从初乳变为成熟乳,在整个哺乳过程中 PG 和 TAG 显着增加。脂肪百分比与脂质浓度的相关性分析表明与八种脂质有很强的相关性(P < 0.05;|r| > 0.80)。在整个哺乳期,质膜磷脂、鞘磷脂或胆固醇酯的丰度没有发现差异。