The hypothesis that apparent ileal digestibility (AID), basal endogenous losses, and standardized ileal digestibility (SID) of AA are not affected by adding acid to collection containers or bags used to collect ileal digesta from pigs was tested. Twenty-four barrows (initial BW: 77.8 kg) that had a T-cannula installed in the distal ileum were fed diets for three 7-d periods. Corn, soybean meal, and wheat middlings were the sole AA sources in each diet and an N-free diet was also used. Within each period, each of the 4 diets was fed to 6 pigs. Among the 6 pigs, digesta from 3 pigs were collected in bags containing no HCl, whereas 40 mL of 3N HCl was included in the bags used to collect digesta from the remaining 3 pigs. Every other bag collected from each pig was emptied into a container without adding HCl, whereas the remaining bags were added to a container along with 40 mL of 3N HCl for each bag. All digesta were stored at –20 °C immediately after collection. Data were first analyzed using a model that included feed ingredient, HCl in bags, HCl in containers, and all 2-way and 3-way interactions as fixed effects. No 3-way interactions were significant, and data were, therefore, analyzed independently for each diet as a 2 × 2 factorial. Results indicated that there were no interactions between adding HCl to collection bags and to containers, and no effects of adding HCl to collection bags or containers for AID, basal endogenous losses, or SID of most AA were observed (Table 1). In conclusion, if digesta are stored at –20 °C immediately after collection, it is not necessary to add acid to digesta collection bags or collection containers.

中文翻译：

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PSII-23 在收集袋或容器中添加氯化氢不会改变饲喂生长猪的玉米、豆粕或小麦中间体中氨基酸的基础内源性损失或消化率

测试了以下假设：AA 的表观回肠消化率 (AID)、基础内源性损失和标准化回肠消化率 (SID) 不受向用于收集猪回肠食糜的收集容器或袋子中添加酸的影响。在回肠末端安装了 T 型套管的 24 只手推车（初始体重：77.8 公斤）被喂食三个 7 天的时期。玉米、豆粕和小麦中间粉是每种日粮中唯一的 AA 来源，并且还使用了无氮日粮。在每个时期内，将 4 种日粮中的每一种饲喂给 6 头猪。在 6 头猪中，3 头猪的食糜被收集在不含 HCl 的袋子中，而用于从其余 3 头猪收集食糜的袋子中装有 40 mL 3N HCl。从每头猪身上收集的每个其他袋子都倒在一个容器中，不添加 HCl，而剩余的袋子与每个袋子的 40 mL 3N HCl 一起添加到容器中。所有食糜在收集后立即储存在 –20 °C。首先使用一个模型分析数据，该模型包括饲料成分、袋装 HCl、容器中的 HCl，以及所有 2-way 和 3-way 相互作用作为固定效应。没有显着的 3 向交互作用，因此，数据作为 2 × 2 因子对每种饮食进行独立分析。结果表明，在收集袋和容器中添加 HCl 之间没有相互作用，在收集袋或容器中添加 HCl 对 AID、基础内源性损失或大多数 AA 的 SID 没有影响（表 1）。总之，如果食糜收集后立即储存在 –20 °C，则无需在食糜收集袋或收集容器中添加酸。