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PSII-16 Effects of Oligosaccharide-based Polymer on Blood Profiles in Weanling Pigs Experimentally Infected with a Pathogenic E. coli
Journal of Animal Science ( IF 3.3 ) Pub Date : 2021-05-08 , DOI: 10.1093/jas/skab054.282
Kwangwook Kim 1 , Yijie He 1 , Cynthia Jinno 1 , Seijoo Yang 1 , Xunde Li 1 , David Bravo 2 , Eric Cox 3 , Yanhong Liu 1
Affiliation  

The objective of this experiment was to investigate dietary supplementation of oligosaccharide-based polymer on blood profiles of weaned pigs experimentally infected with a pathogenic F18 Escherichia coli (E. coli). Forty-eight pigs (7.23 ± 1.11 kg BW) were individually housed in disease containment rooms and randomly allotted to one of four treatments with 12 replicate pigs per treatment. The four dietary treatments were a nursery basal diet (control), and 3 additional diets supplemented with 50 mg/kg Mecadox (AGP), 10 or 20 mg/kg of oligosaccharide-based polymer. The experiment lasted 18 d [7 d before and 11 d after the first inoculation (d 0)]. The doses of F18 E. coli inoculum were 1010 cfu/3 mL oral dose daily for 3 days. Blood samples were collected before E. coli inoculation (d 0), and on d 2, 5, 8, and 11 post-inoculation (PI). Total and differential blood cell count were analyzed by CBC test. All data were analyzed by ANOVA using the PROC MIXED of SAS with pig as the experimental unit. Supplementation of oligosaccharide-based polymer linearly (P < 0.05) reduced white blood cell counts, neutrophils, eosinophils, and basophils on d 2 PI, and neutrophils on d 5 PI, compared with the control. No differences were observed in total and differential white blood cell counts among AGP and two oligosaccharide-based polymer treatments except that pigs fed with AGP had greater (P < 0.05) lymphocytes on d 2 PI compared with pigs fed with oligosaccharide-based polymer diets. Supplementation of low dose oligosaccharide-based polymer or AGP reduced (P < 0.05) red blood cell count and packed cell volume on d 2 PI, whereas inclusion of high dose oligosaccharide-based polymer or AGP reduced (P < 0.05) packed cell volume on d 5 PI, compared with the control. In conclusion, supplementation of oligosaccharide-based polymer may alleviate the systemic inflammation caused by F18 E. coli infection.

中文翻译:

PSII-16 基于寡糖的聚合物对实验性感染致病性大肠杆菌的断奶仔猪血液特征的影响

本实验的目的是研究在实验性感染致病性 F18 大肠杆菌 (E. coli) 的断奶猪的血液特征上添加基于寡糖的聚合物的膳食补充剂。将 48 头猪 (7.23 ± 1.11 kg BW) 单独饲养在疾病控制室中,并随机分配到四种处理中的一种,每次处理 12 头重复猪。四种饮食处理是苗圃基础饮食(对照)和另外 3 种添加 50 mg/kg Mecadox (AGP)、10 或 20 mg/kg 低聚糖基聚合物的饮食。实验持续18 d[第一次接种前7 d,第一次接种后11 d(d 0)]。F18 大肠杆菌接种物的剂量为每天 1010 cfu/3 mL 口服剂量,持续 3 天。在大肠杆菌接种前 (d 0) 和接种后第 2、5、8 和 11 天 (PI) 收集血样。通过CBC测试分析总血细胞计数和分类血细胞计数。所有数据均使用 SAS 的 PROC MIXED 以猪为实验单位,通过 ANOVA 分析。与对照相比,添加基于寡糖的聚合物线性(P<0.05)减少了第 2 天的白细胞计数、中性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞,以及第 5 天的中性粒细胞。在 AGP 和两种基于寡糖的聚合物处理之间没有观察到总和差异白细胞计数的差异,除了与饲喂基于寡糖的聚合物饮食的猪相比,饲喂 AGP 的猪在第 2 天 PI 具有更多 (P < 0.05) 淋巴细胞. 补充低剂量的基于寡糖的聚合物或 AGP 在 d 2 PI 上减少 (P < 0.05) 红细胞计数和压积细胞体积,而与对照相比,包含高剂量基于寡糖的聚合物或AGP在第5天PI减少(P<0.05)堆积的细胞体积。总之,补充基于寡糖的聚合物可以减轻由 F18 大肠杆菌感染引起的全身炎症。
更新日期:2021-05-08
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