To evaluate the multiplicity of reactions to toxic metalloid arsenic (As) with specific emphasis on the role of plant peroxidases, a model plant Nicotiana benthamiana was cultivated in in vitro conditions at various doses of As (applied as As⁵⁺ up to 80 μM). After 28-day cultivation, several physiological characteristics such as plant growth, photosynthetic pigment concentration, As concentration, peroxidase (POX) expression levels, and POX activity were evaluated. A newly sequenced gene for POX has been identified, that belongs to the Class III plant extracellular peroxidases, and its relationship to the genus Solanum as the most relative species has been confirmed. In the control and selected As treatments (20As, 50As, and 80As), newly identified POX expression and POX activity were continuously detected during the whole cultivation period. The plant reactions to As stress were distinguished into three groups: low As, moderate As, and high As. A tight relationship was found between the photosynthetic pigments and POX expression. Accumulation of As in roots and shoots showed correlations with POX activities. The results showed that the diversity of reactions depends on As dose and time exposure and indicate an interface of peroxidase functional role with other physiological processes in plants suffering from As toxicity.

为了评估对有毒准金属砷（As）的反应的多样性，并特别着重于植物过氧化物酶的作用，在体外条件下以各种剂量的As（应用于As ^5+，最高80μM）种植了一种模型植物Nicotiana benthamiana。。培养28天后，评估了一些生理特征，例如植物生长，光合色素浓度，As浓度，过氧化物酶（POX）表达水平和POX活性。已经确定了一种新的POX测序基因，该基因属于III类植物细胞外过氧化物酶，并且与茄属（Solanum）属相关。因为已经确定了相对亲缘种。在对照和选定的砷处理（20As，50As和80As）中，在整个培养期间连续检测到新发现的POX表达和POX活性。植物对砷胁迫的反应分为三组：低砷，中砷和高砷。发现光合色素与POX表达之间存在紧密的关系。根和芽中As的积累表明与POX活性相关。结果表明，反应的多样性取决于砷的剂量和暴露时间，并表明过氧化物酶功能作用与遭受砷毒性的植物中其他生理过程的接口。