Abstract

This study aimed to obtain polyunsaturated fatty acids by hydrolysis of coffee oil catalyzed by Candida rugosa lipase (CRL). The physicochemical properties and fatty acid profile of coffee oil were determined. Oleic (31.09%) and linoleic (51.80%) acids were the major fatty acids in coffee oil. A central composite design was carried out to assess the effect of lipase (0.1–0.5 wt%) and gum Arabic (0–3 wt%) concentrations on free fatty acid formation. Reactions were carried out in 50 mL stirred glass reactors at 37 °C for 2 h using 40 g of medium. CRL was an efficient catalyst of coffee oil hydrolysis, affording hydrolysis yields of 67.1–85.6%.The best predicted concentrations of lipase and gum Arabic were 0.1and 1.5 wt%, respectively. Under these conditions, the hydrolysis yield was 83.1% and the obtained hydrolysate was composed mainly of linoleic and oleic acids.

Graphic Abstract

Coffee oil as a source of omega-6 by enzymatic hydrolysis route

中文翻译：

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基于咖啡油水解的可持续酶法生产必需脂肪酸

摘要

本研究旨在通过皱纹念珠菌催化的咖啡油水解获得多不饱和脂肪酸。脂肪酶（CRL）。测定了咖啡油的理化性质和脂肪酸谱。油酸（31.09％）和亚油酸（51.80％）是咖啡油中的主要脂肪酸。进行了中央复合设计以评估脂肪酶（0.1–0.5 wt％）和阿拉伯树胶（0–3 wt％）浓度对游离脂肪酸形成的影响。使用40 g介质在37°C的50 mL搅拌玻璃反应器中进行反应2小时。CRL是一种有效的咖啡油水解催化剂，水解产率为67.1–85.6％。最佳预测的脂肪酶和阿拉伯树胶浓度分别为0.1和1.5 wt％。在这些条件下，水解产率为83.1％，所得的水解产物主要由亚油酸和油酸组成。

图形摘要

咖啡油通过酶促水解途径作为omega-6的来源