Abstract

The grey type donkeys of Braj region of Uttar Pradesh in India were genetically characterized using ten heterologous microsatellite loci. At these loci the PCR product size ranged from 75-95 bp at locus HTG6 to 251-277 bp at locus COR18. The observed number of alleles varied from 4 (HTG15) to 10 (HTG7 and AHT4) with a mean of 7.50 ± 1.96. The observed heterozygosity ranged from 0.46 (HTG6 and NVHEQ54) to 0.79 (COR18) with a mean of 0.66 ± 0.12. The mean genetic diversity estimate (FIS) was 0.157. When these donkeys were compared on the basis of allelic frequency data at these loci to the brown type donkeys of Ladakh, Spiti and Rayalseema regions in the union territories/states of Ladakh, Himachal Pradesh and Andhra Pradesh, respectively, they clustered independently from these three donkey populations in a dendrogram based on Goldstein’s average square distances indicating their genetic distinctness.

摘要

使用十个异源微卫星基因座对印度北方邦Braj地区的灰色型驴进行了遗传表征。在这些位点，PCR 产物大小范围从位点 HTG6 的 75-95 bp 到位点 COR18 的 251-277 bp。观察到的等位基因数量从 4 (HTG15) 到 10 (HTG7 和 AHT4) 不等，平均值为 7.50 ± 1.96。观察到的杂合性范围为 0.46（HTG6 和 NVHEQ54）至 0.79（COR18），平均值为 0.66 ± 0.12。平均遗传多样性估计值（FIS) 为 0.157。当根据这些位点的等位基因频率数据将这些驴分别与拉达克、喜马偕尔邦和安得拉邦联合领土/州的拉达克、斯皮蒂和拉亚尔西马地区的棕色型驴进行比较时，它们独立于这三个基于 Goldstein 的平均平方距离的树状图中的驴种群表明它们的遗传差异。