Industrial Crops and Products ( IF 5.9 ) Pub Date : 2021-05-07 , DOI: 10.1016/j.indcrop.2021.113595 Marcos Vinicius Silva de Andrade , Renato Delmondez de Castro , Diego da Silva Cunha , Valdir Gomes Neto , Maria Gabriela Aparecida Carosio , Antonio Gilberto Ferreira , Lourdes Cardoso de Souza-Neta , Luzimar Gonzaga Fernandez , Paulo Roberto Ribeiro
Stevia rebaudiana is an important industrial crop due to the accumulation of high amounts of steviol glycosides (SG - natural sweeteners) in its leaves. S. rebaudiana cultivation has faced some pushbacks since this species is highly responsive to environmental factors, such as light availability. Sixty days after sowing, plants were transferred to different photoperiod conditions (12/12 h, 15/9 h, and 16/8 h of light/dark). Leaf extracts of plants growing at the 16/8 h photoperiod showed greater accumulation of antioxidant-like metabolites as compared to the other two photoperiods, which might be explained by the total phenolic content of the extracts. Additionally, plants growing at the 16/8 h photoperiod showed increased SOD activity as compared to plants growing at the 15/9 h photoperiod, which in turn showed higher SOD activity than plants growing at the 12/12 h photoperiod. It seems that SOD isoforms act synergically with phenolic compounds to prevent possible damages caused by reactive oxygen species that are produced in plants growing at long-day photoperiods. Sixteen metabolites were identified by Nuclear Magnetic Resonance in the leaf and stem extracts. Alanine, formate, choline, kaempferol-3-O-β-d-glucopyranoside-7-O-α-l-rhamnopyranoside, and gallic acid seems to contribute to maintain stevia homeostasis under unfavorable conditions. Furthermore, the accumulation of SGs and other bioactive compounds in S. rebaudiana in response to different photoperiods provides important leads for the improvement of its large-scale cultivation, as well as for the extraction and purification of phytochemicals with industrial interest.
中文翻译:
在不同的光周期条件下栽培的甜叶菊(Bertoni):改善生理和生化特性以用于工业应用
甜叶菊是重要的工业作物,因为其叶片中积累了大量的甜菊醇糖苷(SG-天然甜味剂)。葡萄球菌由于该物种对环境因素(例如光的可用性)具有高度的响应能力,因此种植面临着一些挫折。播种六十天后,将植物转移到不同的光周期条件下(明/暗12/12小时,15/9小时和16/8小时)。与其他两个光周期相比,在16/8 h光周期下生长的植物的叶提取物显示出更大的抗氧化剂样代谢产物积累,这可以用提取物中的总酚含量来解释。另外,与在15 / 9h光周期下生长的植物相比,在16 / 8h光周期下生长的植物显示出增加的SOD活性,这又比在12 / 12h光周期下生长的植物显示出更高的SOD活性。似乎SOD亚型与酚类化合物具有协同作用,以防止由在长期光周期下生长的植物中产生的活性氧引起的可能损害。通过核磁共振法在叶和茎提取物中鉴定出16种代谢物。丙氨酸,甲酸盐,胆碱,山奈酚-3-O-β-d-吡喃葡萄糖苷-7-O-α- 1-鼠李糖吡喃糖苷和没食子酸似乎有助于在不利条件下维持甜菊稳态。此外,响应于不同的光周期,SGs和其他生物活性化合物在雷氏链球菌中的积累为改进其大规模栽培以及具有工业兴趣的植物化学物质的提取和纯化提供了重要的线索。