A Bacillus siamensis YC-9 strain was isolated from termite nest. The strain was used to produce several important industrial enzymes by utilizing different agricultural by-products with solid-state fermentation (SSF). It was observed that most of the substrates that were studied were strongly positively affected by alkaline treatment for the production of enzymes. The highest yields for protease, α-amylase, and cellulase were 82.87, 42.81, and 1.54 IU g⁻¹, achieved on alkaline-treated corn pericarp, rice bran, and wheat bran respectively. However, untreated soybean meal was the best choice for the secretion of pectinases (66.65 IU g⁻¹). In general, ultrasonic pretreatment was more effective than microwave treatment and it promoted the production of proteases on soybean meal, sunflower meal, and wheat bran. The findings of the present study will provide some theoretical basis for enzyme production in the future. © 2021 Society of Industrial Chemistry and John Wiley & Sons Ltd.

中文翻译：

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与白蚁巢相关的暹罗芽孢杆菌YC-9在农业副产品固态发酵中生产胞外酶

甲杆菌siamensis等YC-9菌株是从白蚁巢隔离。该菌株用于通过固态发酵 (SSF) 利用不同的农业副产品来生产几种重要的工业酶。据观察，所研究的大多数底物都受到碱处理产生酶的强烈积极影响。蛋白酶、α-淀粉酶和纤维素酶的最高产量分别为82.87、42.81 和 1.54 IU g ^-1，分别在碱处理的玉米果皮、米糠和麦麸上实现。然而，未经处理的豆粕是果胶酶分泌的最佳选择（66.65 IU g ^-1）。总的来说，超声波预处理比微波处理更有效，它促进了豆粕、葵花粕和麦麸中蛋白酶的产生。本研究结果将为今后的酶生产提供一定的理论依据。© 2021 工业化学学会和 John Wiley & Sons Ltd.