This study aimed to investigate the effect of dietary tetramethylpyrazine (TMP) on the growth performance, nutrient digestion, blood parameters and immunity of broilers under oxidative stress. Five treatments included negative control, positive control with lipopolysaccharide induction and TMP addition at 50, 100 and 150 mg/kg of diet using 600 male Arbor Acres broiler chicks. Results showed that during 1–14 days of age, body weight gain and feed efficiency in the positive control were worsened (p < .05) compared with the negative control, while with incremental TMP doses from 0 to 150 mg/kg there were linear and quadratic increases (p < .001) in body weight gain and a linear decrease in feed/gain (p = .001). During 12–14 days of age, with incremental TMP doses, crude protein digestibility was linearly increased (p = .001), and gross energy utilization was linearly and quadratically changed (p < .001). At 14 days of age, the TMP beneficially regulated digestive enzymes, blood parameters and immunoglobulins, showing linear and quadratic responses (p ≤ .008) on trypsin, lipase, glutamic pyruvic transaminase, glucose, lipoproteins, albumin, immunoglobulins (M, Y), interleukin 6 and interferon α, and only linear changes (p ≤ .030) on amylase, glutamic oxaloacetic transaminase, immunoglobulin A and interleukin 2. Most parameters in TMP groups reached to the levels of negative control and the effects of TMP at 100 or 150 mg/kg were more pronounced on body weight gain, crude protein digestibility, trypsin and glutamic pyruvic transaminase. It is concluded that TMP can be used as a feed additive capable of improving growth, blood parameter and immunity of broiler chicks under oxidative stress.

中文翻译：

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补充四甲基吡嗪可改善暴露于氧化应激的肉鸡的生产性能、消化、血液和免疫状态

本研究旨在探讨日粮四甲基吡嗪（TMP）对氧化应激下肉鸡生长性能、营养消化、血液参数和免疫力的影响。五种处理包括阴性对照、脂多糖诱导阳性对照和使用 600 只雄性 Arbor Acres 肉鸡以 50、100 和 150 mg/kg 饮食添加 TMP。结果表明，在 1-14 日龄期间，与阴性对照相比，阳性对照的体重增加和饲料效率恶化 ( p  < .05)，而随着 TMP 剂量从 0 到 150 mg/kg 的增加，存在线性 体重增加呈二次增加（p < .001），饲料/增重呈线性下降（ p = .001)。在 12-14 日龄期间，随着 TMP 剂量的增加，粗蛋白消化率线性增加（p  = .001），总能量利用呈线性和二次变化（p  < .001）。在 14 日龄时，TMP 有益地调节消化酶、血液参数和免疫球蛋白，对胰蛋白酶、脂肪酶、谷氨酸丙酮酸转氨酶、葡萄糖、脂蛋白、白蛋白、免疫球蛋白 (M、Y) 显示出线性和二次反应 ( p  ≤ .008) , 白细胞介素 6 和干扰素 α, 只有线性变化 ( p ≤ .030) 对淀粉酶、谷氨酸草酰乙酸转氨酶、免疫球蛋白 A 和白细胞介素 2 的影响。TMP 组中的大多数参数达到阴性对照的水平，并且 TMP 在 100 或 150 mg/kg 对体重增加的影响更明显，粗蛋白质消化率、胰蛋白酶和谷氨酸丙酮酸转氨酶。得出的结论是，TMP可以作为一种饲料添加剂，提高氧化应激下肉鸡的生长、血液参数和免疫力。