Little information is available on differences between maternally and artificially incubated eggs in the economically important crayfish Procambarus clarkii. Brood care in this species is expected to exert effects on the eggs. In this work, we evaluated the hatching rates of artificially incubated eggs, without brood care, compared to those maternally incubated through different development stages. We also examined whether the maternal-offspring connections in ovigerous females were related to embryonic development at the physiological level based on comparative proteomic analysis. The experiments led to two key results. First, hatching rates increased continuously throughout the nine distinct development phases when detaching maternally incubated eggs from the mothers' pleopods individually. Further, a critical period of artificially incubated eggs was observed around the gastrula stage, with the hatching rate ranging from approx. 24% to 45%. Second, blood plasma proteome profiles differed significantly between ovigerous females and aborted females during the gastrula stage (p < 0.05). Of the identified 27 differentially expressed proteins, 23 were significantly up-regulated, whereas four were down-regulated (p < 0.05). Functions of DEPs were linked to the binding, regulation, and immune response, particularly in two strongly expressed PGDH-like and CAT-like proteins (fold-change>3.0). These results suggested that the dynamic changes of plasma proteins in ovigerous females might be related to early isolated egg anti-infectivity and hence affect the hatching success. Yet, the mechanisms behind the encountered relationships need further exploration. In summary, we presented the first evidence of proteomic changes in ovigerous female plasma, revealing that brood care in P. clarkii may include more than physical behavior

关于经济上重要的小龙虾Procambarus clarkii的母体和人工孵化的卵之间的差异的信息很少。。预计该物种的育雏会对卵产生影响。在这项工作中，我们评估了未经育雏的人工孵化的卵与通过不同发育阶段母体孵化的卵的孵化率。根据比较蛋白质组学分析，我们还检查了雌雄同体的母子后代在生理水平上是否与胚胎发育有关。实验得出了两个关键结果。首先，当从母亲的腹足中单独取出母体孵化的卵时，在九个不同的发育阶段中，孵化率不断提高。此外，在腹果期周围观察到人工孵化卵的关键时期，其孵化率范围大约为1-10℃。24％至45％。第二，p  ＜0.05）。在鉴定出的27种差异表达蛋白中，有23种显着上调，而4种下调（p  <0.05）。DEPs的功能与结合，调节和免疫反应有关，特别是在两种强烈表达的PGDH样和CAT样蛋白中（倍数变化> 3.0）。这些结果表明，卵子雌性中血浆蛋白的动态变化可能与早期分离的卵的抗感染性有关，因此影响了孵化的成功。但是，所遇到的关系背后的机制需要进一步探索。总而言之，我们提出了卵生雌性血浆蛋白质组学变化的第一个证据，揭示了克拉克氏疟原虫的育雏护理可能不仅包括身体行为