As environmental temperatures continue to rise, heat stress (HS) is having a negative effect on the livestock industry. In order to solve this problem, many studies have been conducted to reduce HS. Among them, early heat exposure has been suggested as a method for reducing HS in poultry. In this study, we analyzed proteomics and tried to identify the metabolic mechanisms of early heat exposure on acute HS. A total of 48 chicks were separated into three groups: CC (control groups raised at optimum temperature), CH (raised with CC but exposed acute HS at the 35th day), and HH (raised with CC but exposed early heat at the fifth day and acute HS at the 35th day). After the whole period, liver samples were collected for proteomic analysis. A total of 97 differentially expressed proteins were identified by acute HS. Of these, 62 proteins recovered their expression levels by early heat exposure. We used these 62 proteins to determine the protective effects of early heat exposure. Of the various protein-related terms, we focused on the oxidative phosphorylation, fatty acid metabolism, carbohydrate metabolism, and energy production metabolism. Our findings suggest the possibility of early heat exposure effects in acute HS that may be useful in breeding or management techniques for producing broilers with high heat resistance.

中文翻译：

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早期热暴露对急性热应激下肉鸡肝脏蛋白质组学的影响

随着环境温度的持续升高，热应激（HS）对畜牧业产生了负面影响。为了解决这个问题，已经进行了许多研究来降低HS。在它们之中，已经建议早期热暴露作为降低家禽HS的方法。在这项研究中，我们分析了蛋白质组学，并试图确定急性HS早期受热的代谢机制。总共48只小鸡分为三组：CC（对照组在最佳温度下饲养），CH（CC饲养，但在第35天暴露于急性HS）和HH（CC饲养，但在第5天暴露于早期热量）和第35天的急性HS）。在整个时期后，收集肝脏样本进行蛋白质组学分析。急性HS总共鉴定出97种差异表达的蛋白质。这些，通过早期热暴露，有62种蛋白质恢复了它们的表达水平。我们使用这62种蛋白质来确定早期受热的保护作用。在与蛋白质相关的各种术语中，我们集中于氧化磷酸化，脂肪酸代谢，碳水化合物代谢和能量产生代谢。我们的发现表明，急性HS早期受热影响的可能性可能对生产高耐热性肉鸡的育种或管理技术有用。