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The seed-associated microbiome of four cactus species from Southern Chihuahuan Desert
Journal of Arid Environments ( IF 2.7 ) Pub Date : 2021-05-08 , DOI: 10.1016/j.jaridenv.2021.104531
Ernesto Mascot-Gómez , Joel Flores , Nguyen E. López-Lozano

One of the riskiest stages in the reproduction of cactus is the germination of seeds. Although evidence indicates the relevance of the seed-associated microbiome during this stage, little is known about its structure and function. In this work, we explore the diversity of the seed-associated microbiome of cactus, their role in the germination and protection of the emerging embryo. We characterized the microbial communities of the internal tissues (endoseminal) and those that colonize the surface of the seeds (episeminal) of four cactus species (Echinocactus platyacanthus, Ferocactus latispinus, Ferocactus pilosus, and Stenocereus queretaroensis). We sequenced by Illumina the 16S rRNA gene for Bacteria and the ITS1/ITS4 region for fungi. We isolated cultivable microorganisms and characterized their plant growth-promoting abilities. We determined that microbial communities associated with cactus seeds have high richness but are dominated by few taxa, and in most cases, there are clear differences between episeminal and endoseminal microbial groups, especially for fungi. The cactus seed microbiota comprises potential pathogens and beneficial microorganisms that could influence the germination percentage and seedling's development. We can highlight bacterial groups such as Kluyvera, Bacillus, Paenibacillus, and Stenotrophomonas; for fungi, the classes Tremellomycetes, Dothideomycetes, Eurotiomycetes, Leotiomycetes, and Sordariomycetes.



中文翻译:

奇瓦南沙漠南部四种仙人掌物种的种子相关微生物组

仙人掌繁殖中最危险的阶段之一是种子发芽。尽管有证据表明在此阶段与种子相关的微生物组具有相关性,但对其结构和功能知之甚少。在这项工作中,我们探索了与种子相关的仙人掌的微生物组的多样性,它们在萌发和保护新兴胚中的作用。我们表征了内部组织(内膜)的微生物群落和定居在四个仙人掌物种(棘皮E,Fer铁腕,oc皮毛虫Ste皮球菌)种子表面(表皮)的微生物群落的特征。)。我们通过Illumina对细菌的16S rRNA基因和对真菌的ITS1 / ITS4区进行了测序。我们分离出可培养的微生物,并表征了它们促进植物生长的能力。我们确定与仙人掌种子相关的微生物群落丰富度很高,但几乎没有分类单元,而且在大多数情况下,尤其是对于真菌而言,在表观微生物和内寄生微生物之间存在明显的差异。仙人掌种子微生物群包含潜在的病原体和有益的微生物,这些微生物可能会影响发芽率和幼苗的发育。我们可以突出显示细菌组,例如克鲁维拉杆菌,芽孢杆菌,芽孢杆菌嗜单胞菌;对于真菌而言,有类真菌,类真菌,欧洲真菌,类真菌Sordariomycetes

更新日期:2021-05-08
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