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Sensitivity of rice to photooxidation associated with antioxidant enzyme activity and carbon assimilation
Acta Physiologiae Plantarum ( IF 2.6 ) Pub Date : 2021-05-07 , DOI: 10.1007/s11738-021-03260-y
Zeng Yanda , Zhang Qijun , Xiao Enxing , Li Xia , Lv Chuangen

In this study, rice cultivar 8272 (yellowing leaves) was compared with cultivar 9311 (normal leaf color) to clarify the mechanism underlying the chlorosis. The 8272 leaf color was sensitive to intense sunlight accompanied by high temperatures, which induced the yellowing of leaves, whereas the 9311 leaf color was normal under the same growing conditions. A shading experiment in the field and an artificial climate chamber test revealed that the chlorosis of 8272 leaves was due to high-intensity light, rather than high temperatures. Analyses of chlorophyll fluorescence parameters indicated that the photosystem II (PSII) reaction center activity of 8272N (grown under natural high light conditions) was significantly lower than that of 8272S (under shade), with the electron transfer significantly inhibited. The H2O2, O2.−, and malondialdehyde (MDA) contents increased sharply in 8272N, and the antioxidant enzymes were also activated. A comparison of the enriched KEGG pathways among the differentially expressed genes (DEGs) between 8272 and 9311 indicated genes related to abiotic stress tolerance were highly expressed in 8272N. In contrast, the expression levels of the key genes in the carbon assimilation metabolic pathway were significantly lower in 8272N than in 9311N. A qRT-PCR analysis confirmed the results of the RNA-seq and antioxidant enzyme activity analyses. Thus, the chlorosis of 8272 leaves in response to intense light may be associated with the mechanisms contributing to the photosynthetic control of electron transport via carbon assimilation in leaves.



中文翻译:

水稻对光氧化的敏感性与抗氧化酶活性和碳同化作用有关

在这项研究中,将水稻8272品种(黄叶)与9311品种(正常叶色)进行了比较,以阐明绿化的潜在机理。8272叶子的颜色对强烈的阳光和高温敏感,高温导致叶片发黄,而9311叶子的颜色在相同的生长条件下是正常的。野外的遮荫试验和人工气候箱试验表明,8272叶片的褪绿是由于高强度光而不是高温引起的。叶绿素荧光参数的分析表明,8272N(在自然高光条件下生长)的光系统II(PSII)反应中心活性显着低于8272S(在阴影下),并且电子转移受到明显抑制。H 2 O2,O 2 .-和丙二醛(MDA)含量在8272N中急剧增加,并且抗氧化酶也被激活。比较了8272和9311之间差异表达基因(DEG)之间富集的KEGG途径,表明与非生物胁迫耐受性相关的基因在8272N中高度表达。相反,碳同化代谢途径中关键基因的表达水平在8272N中显着低于9311N。qRT-PCR分析证实了RNA-seq和抗氧化酶活性分析的结果。因此,8272叶片对强光的减绿作用可能与通过叶片中碳同化作用促进光合作用控制电子传递的机制有关。

更新日期:2021-05-08
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