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Amidation-Modified Apelin-13 Regulates PPARγ and Perilipin to Inhibit Adipogenic Differentiation and Promote Lipolysis
Bioinorganic Chemistry and Applications ( IF 3.8 ) Pub Date : 2021-05-08 , DOI: 10.1155/2021/3594630
Sha Wang 1 , Guoying Gao 2 , Yiwei He 2 , Qiong Li 1 , Zhan Li 2 , Guoxiang Tong 1
Affiliation  

With the adjustment of human diet and lifestyle changes, the prevalence of obesity is increasing year by year. Obesity is closely related to the excessive accumulation of white adipose tissue (WAT), which can synthesize and secrete a variety of adipokines. Apelin is a biologically active peptide in the adipokines family. Past studies have shown that apelin plays an important regulatory role in the pathogenesis and pathophysiology of diseases such as the cardiovascular system, respiratory system, digestive system, nervous system, and endocrine system. Apelin is also closely related to diabetes and obesity. Therefore, we anticipate that apelin-13 has an effect on lipometabolism and intend to explore the effect of apelin-13 on lipometabolism at the cellular and animal levels. In in vitro experiments, amidation-modified apelin-13 can significantly reduce the lipid content; TG content; and the expression of PPARγ, perilipin mRNA, and protein in adipocytes. Animal experiments also show that amidation modification apelin-13 can improve the abnormal biochemical indicators of diet-induced obesity (DOI) rats and can reduce the average diameter of adipocytes in adipose tissue, the concentration of glycerol, and the expression of PPARγ and perilipin mRNA and protein. Our results show that apelin-13 can affect the metabolism of adipose tissue, inhibit adipogenic differentiation of adipocytes, promote lipolysis, and thereby improve obesity. The mechanism may be regulating the expression of PPARγ to inhibit adipogenic differentiation and regulating the expression of perilipin to promote lipolysis. This study helps us understand the role of apelin-13 in adipose tissue and provide a basis for the elucidation of the regulation mechanism of lipometabolism and the development of antiobesity drugs.

中文翻译:

酰胺化修饰的Apelin-13调节PPARγ和Perilipin抑制成脂分化并促进脂解

随着人类饮食的调整和生活方式的改变,肥胖症的患病率逐年增加。肥胖与白色脂肪组织(WAT)的过度积累密切相关,后者可以合成和分泌多种脂肪因子。Apelin是脂肪因子家族中的一种生物活性肽。过去的研究表明,apelin在诸如心血管系统,呼吸系统,消化系统,神经系统和内分泌系统等疾病的发病机理和病理生理中起着重要的调节作用。Apelin也与糖尿病和肥胖症密切相关。因此,我们预期apelin-13对脂肪代谢有影响,并打算在细胞和动物水平上探索apelin-13对脂肪代谢的影响。在体外实验中 酰胺化修饰的apelin-13可以显着降低脂质含量;TG含量 和PPAR的表达脂肪细胞中的γ,周脂素mRNA和蛋白。动物实验还表明,酰胺化修饰Apelin水平-13可以改善饮食诱发的肥胖症的异常生化指标(DOI)的大鼠,并能降低脂肪细胞的脂肪组织中的平均直径,甘油的浓度,和PPAR的表达γ和围脂滴蛋白mRNA和蛋白质。我们的结果表明,apelin-13可以影响脂肪组织的代谢,抑制脂肪细胞的脂肪形成分化,促进脂肪分解,从而改善肥胖症。该机构可被调节PPAR的表达γ抑制成脂分化,调节周脂素的表达,促进脂肪分解。这项研究有助于我们了解apelin-13在脂肪组织中的作用,并为阐明脂肪代谢的调控机制和开发抗肥胖药提供基础。
更新日期:2021-05-08
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