Following fusion between two or more individual cells, the resulting cellular entity must undergo extensive restructuring of its plasma membrane and cytoskeleton in order to maintain its mechanical and physiological function. In artificial cell fusion that is executed by external triggering, such restructuring could be problematic due to the absence of preconditioning biological signals. In this work we study the reorganization of the actin filaments in adenocarcinoma cells that were fused using plasmonic triggering, i.e. the irradiation by resonant femtosecond laser pulses of cells specifically targeted by gold nanoparticles. Time-lapse confocal microscopy of the fusing cells has revealed the formation of large-scale actin networks that preserve the local orientations of the original actin cytoskeletons. The results confirm the local nature of the plasmonic interactions that were confined to the cells’ plasma membranes and would help studying the development and dynamics of actin networks by offering a relatively stable, living cellular environment that supports large-scale actin growth.

中文翻译：

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等离子细胞融合后大细胞内肌动蛋白网络的形成

两个或多个单个细胞融合后，生成的细胞实体必须对其质膜和细胞骨架进行广泛的重组，以维持其机械和生理功能。在由外部触发执行的人工细胞融合中，由于缺乏预处理生物信号，这种重组可能会出现问题。在这项工作中，我们研究了使用等离子体触发融合的腺癌细胞中肌动蛋白丝的重组，即由金纳米粒子特异性靶向的细胞的共振飞秒激光脉冲照射。融合细胞的延时共聚焦显微镜揭示了大规模肌动蛋白网络的形成，这些网络保留了原始肌动蛋白细胞骨架的局部方向。