Super-resolution microscopy (SRM) comprises a suite of techniques well-suited to probing the nanoscale landscape of genomic function and dysfunction. Offering the specificity and sensitivity that has made conventional fluorescence microscopy a cornerstone technique of biological research, SRM allows for spatial resolutions as good as 10 nanometers. Moreover, single molecule localization microscopies (SMLMs) enable examination of individual molecular targets and nanofoci allowing for the characterization of subpopulations within a single cell. This review describes how key advances in both SRM techniques and sample preparation have enabled unprecedented insights into DNA structure and function, and highlights many of these new discoveries. Ongoing development and application of these novel, highly interdisciplinary SRM assays will continue to expand the toolbox available for research into the nanoscale genomic landscape.

中文翻译：

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通过超高分辨率荧光显微镜了解DNA的组织，损伤和修复。

超分辨率显微镜（SRM）包含一套非常适合探测基因组功能和功能异常的纳米级景观的技术。由于提供了使常规荧光显微镜成为生物学研究的基础技术的特异性和敏感性，SRM的空间分辨率高达10纳米。此外，单分子定位显微镜检查（SMLM）可以检查单个分子靶标和纳米焦点，从而可以表征单个细胞内的亚群。这篇综述描述了SRM技术和样品制备方面的关键进展如何使人们对DNA结构和功能有了空前的见识，并着重介绍了许多这些新发现。这些小说的持续开发和应用，